Literature DB >> 18476725

PLP catabolism: identification of the 2-(Acetamidomethylene)succinate hydrolase gene in Mesorhizobium loti MAFF303099.

Tathagata Mukherjee1, David G Hilmey, Tadhg P Begley.   

Abstract

The function of the mlr6787 gene from Mesorhizobium loti MAFF303099 has been identified. This gene encodes 2-(acetamidomethylene)succinate hydrolase, an enzyme involved in the catabolism of pyridoxal 5'-phosphate (vitamin B 6). This enzyme was overexpressed in Escherichia coli, purified to homogeneity, and characterized. 2-(Acetamidomethylene)succinate hydrolase catalyzes the hydrolysis of 2-(acetamidomethylene)succinate to yield succinic semialdehyde, acetic acid, carbon dioxide, and ammonia. The k cat and K M for this reaction were 0.6 s (-1) and 143 microM, respectively. The enzyme was shown to utilize the E isomer of 2-(acetamidomethylene)succinate.

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Year:  2008        PMID： 18476725     DOI： 10.1021/bi8004158

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  5 in total

1.  Elucidation of the trigonelline degradation pathway reveals previously undescribed enzymes and metabolites.

Authors:  Nadia Perchat; Pierre-Loïc Saaidi; Ekaterina Darii; Christine Pellé; Jean-Louis Petit; Marielle Besnard-Gonnet; Véronique de Berardinis; Maeva Dupont; Alexandra Gimbernat; Marcel Salanoubat; Cécile Fischer; Alain Perret
Journal:  Proc Natl Acad Sci U S A       Date:  2018-04-23       Impact factor: 11.205

2.  Structure determination and characterization of the vitamin B6 degradative enzyme (E)-2-(acetamidomethylene)succinate hydrolase.

Authors:  Kathryn M McCulloch; Tathagata Mukherjee; Tadhg P Begley; Steven E Ealick
Journal:  Biochemistry       Date:  2010-02-16       Impact factor: 3.162

3.  Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway I of pyridoxine.

Authors:  Jun Kobayashi; Hiromi Yoshida; Huy Nhat Chu; Yu Yoshikane; Shigehiro Kamitori; Toshiharu Yagi
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-07-30

4.  Structure of the PLP degradative enzyme 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti MAFF303099 and its mechanistic implications.

Authors:  Kathryn M McCulloch; Tathagata Mukherjee; Tadhg P Begley; Steven E Ealick
Journal:  Biochemistry       Date:  2009-05-19       Impact factor: 3.162

5.  How does (E)-2-(acetamidomethylene)succinate bind to its hydrolase? From the binding process to the final result.

Authors:  Ji-Long Zhang; Qing-Chuan Zheng; Zheng-Qiang Li; Hong-Xing Zhang
Journal:  PLoS One       Date:  2013-01-07       Impact factor: 3.240
  5 in total

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