M E Rupp1, J Han, R V Goering. 1. Department of Internal Medicine University of Nebraska Medical Center 600 S. 42nd Street, Omaha, NE 68198-5400, USA.
Abstract
OBJECTIVE: The purpose of this study was to determine whether colonization was persistent or recurrent in a small group of women who had repeated recovery of Staphylococcus saprophyticus from their urogenital tracts. METHODS: Paired isolates of S. saprophyticus from each of the study subjects were genotypically typed by plasmid fingerprinting and comparison of chromosomal-DNA restriction fragment-length polymorphism patterns by field-inversion gel electrophoresis (FIGE) and contour-clamped homogenous electric-field (CHEF) electrophoresis. RESULTS: All isolates of S. saprophyticus from the study subjects were classified as genetically unique by each of the typing methods. CONCLUSIONS: The subjects experienced recurrent colonization with different isolates of S. saprophyticus. These findings may have broader implications regarding the pathogenesis and recurrence of S. saprophyticus urinary-tract infection.
OBJECTIVE: The purpose of this study was to determine whether colonization was persistent or recurrent in a small group of women who had repeated recovery of Staphylococcus saprophyticus from their urogenital tracts. METHODS: Paired isolates of S. saprophyticus from each of the study subjects were genotypically typed by plasmid fingerprinting and comparison of chromosomal-DNA restriction fragment-length polymorphism patterns by field-inversion gel electrophoresis (FIGE) and contour-clamped homogenous electric-field (CHEF) electrophoresis. RESULTS: All isolates of S. saprophyticus from the study subjects were classified as genetically unique by each of the typing methods. CONCLUSIONS: The subjects experienced recurrent colonization with different isolates of S. saprophyticus. These findings may have broader implications regarding the pathogenesis and recurrence of S. saprophyticusurinary-tract infection.