Literature DB >> 1847389

Efficient purification of recombinant human tumor necrosis factor beta from Escherichia coli yields biologically active protein with a trimeric structure that binds to both tumor necrosis factor receptors.

H J Schoenfeld1, B Poeschl, J R Frey, H Loetscher, W Hunziker, A Lustig, M Zulauf.   

Abstract

A fast and efficient method for medium scale purification of recombinant human tumor necrosis factor beta (rTNF-beta) from Escherichia coli cells is described. The purified rTNF-beta displayed biological activity similar to rTNF-alpha in a WEHI 164 cell cytotoxicity assay. The titration curve of rTNF-beta and elution profiles of rTNF-beta in gel filtration experiments were different from those of rTNF-alpha. However, light scattering and ultra-centrifugation studies showed that both cytokines have trimeric structures in solution at 0.5 mg/ml, with minor differences in the distribution of nontrimeric species. rTNF-beta bound to purified 55- and 75-kDa TNF receptors with high affinity. The binding of rTNF-beta to either receptor was analyzed on Scatchard plots and compared with that of rTNF-alpha.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1847389

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  13 in total

1.  Oligomeric tumour necrosis factor alpha slowly converts into inactive forms at bioactive levels.

Authors:  A Corti; G Fassina; F Marcucci; E Barbanti; G Cassani
Journal:  Biochem J       Date:  1992-06-15       Impact factor: 3.857

2.  Molecular cloning and expression of the mouse Tnf receptor type b.

Authors:  J G Rothe; M Brockhaus; R Gentz; W Lesslauer
Journal:  Immunogenetics       Date:  1991       Impact factor: 2.846

3.  Mutual antagonism between interferon-gamma and tumor necrosis factor-alpha on fibroblast-like synoviocytes: paradoxical induction of IFN-gamma and TNF-alpha receptor expression.

Authors:  J M Alvaro-Gracia; C Yu; N J Zvaifler; G S Firestein
Journal:  J Clin Immunol       Date:  1993-05       Impact factor: 8.317

Review 4.  Pathogenesis of malaria and clinically similar conditions.

Authors:  Ian A Clark; Lisa M Alleva; Alison C Mills; William B Cowden
Journal:  Clin Microbiol Rev       Date:  2004-07       Impact factor: 26.132

5.  Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides.

Authors:  Joseph J Bellucci; Miriam Amiram; Jayanta Bhattacharyya; Dewey McCafferty; Ashutosh Chilkoti
Journal:  Angew Chem Int Ed Engl       Date:  2013-02-19       Impact factor: 15.336

Review 6.  Tumor necrosis factor receptors--structure and function.

Authors:  J Rothe; G Gehr; H Loetscher; W Lesslauer
Journal:  Immunol Res       Date:  1992       Impact factor: 2.829

7.  Binding of cytokines to pharmaceutically prepared human immunoglobulin.

Authors:  M Svenson; M B Hansen; K Bendtzen
Journal:  J Clin Invest       Date:  1993-11       Impact factor: 14.808

8.  Human lymphotoxin mutein lacks hypotensive activity but has higher in vivo antitumor activity than lymphotoxin or tumor necrosis factor.

Authors:  M Taniyama; T Morita; Y Yamagishi; A Kato; C Bando; N Okawa; A Kaji
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-01       Impact factor: 11.205

9.  A wake-like state in vitro induced by transmembrane TNF/soluble TNF receptor reverse signaling.

Authors:  Cheryl Dykstra-Aiello; Khia Min Sabrina Koh; Joseph Nguyen; Mengran Xue; Sandip Roy; James M Krueger
Journal:  Brain Behav Immun       Date:  2021-02-09       Impact factor: 7.217

10.  Atomic Scale Interactions between RNA and DNA Aptamers with the TNF-α Protein.

Authors:  Homayoun Asadzadeh; Ali Moosavi; Georgios Alexandrakis; Mohammad R K Mofrad
Journal:  Biomed Res Int       Date:  2021-07-16       Impact factor: 3.411
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.