Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides.

× No keyword cloud information.

23 in total

1. Purification of recombinant proteins by fusion with thermally-responsive polypeptides.

Authors: D E Meyer; A Chilkoti
Journal: Nat Biotechnol Date: 1999-11 Impact factor: 54.908

2. Sortase-catalyzed transformations that improve the properties of cytokines.

Authors: Maximilian W Popp; Stephanie K Dougan; Tzu-Ying Chuang; Eric Spooner; Hidde L Ploegh
Journal: Proc Natl Acad Sci U S A Date: 2011-02-04 Impact factor: 11.205

3. Calculation of protein extinction coefficients from amino acid sequence data.

Authors: S C Gill; P H von Hippel
Journal: Anal Biochem Date: 1989-11-01 Impact factor: 3.365

4. Proteome-scale purification of human proteins from bacteria.

Authors: Pascal Braun; Yanhui Hu; Binghua Shen; Allison Halleck; Malvika Koundinya; Ed Harlow; Joshua LaBaer
Journal: Proc Natl Acad Sci U S A Date: 2002-03-05 Impact factor: 11.205

5. Association of lysosomal activity with sensitivity and resistance to tumor necrosis factor in murine L929 cells.

Authors: J D Liddil; R T Dorr; P Scuderi
Journal: Cancer Res Date: 1989-05-15 Impact factor: 12.701

6. Comparison of in vitro cell cytotoxic assays for tumor necrosis factor.

Authors: D A Flick; G E Gifford
Journal: J Immunol Methods Date: 1984-03-30 Impact factor: 2.303

7. Heterotrimers formed by tumor necrosis factors of different species or muteins.

Authors: P Ameloot; W Declercq; W Fiers; P Vandenabeele; P Brouckaert
Journal: J Biol Chem Date: 2001-05-22 Impact factor: 5.157

8. Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA.

Authors: Ryan G Kruger; Balint Otvos; Brenda A Frankel; Matthew Bentley; Patrick Dostal; Dewey G McCafferty
Journal: Biochemistry Date: 2004-02-17 Impact factor: 3.162

9. Sortase-mediated protein ligation: a new method for protein engineering.

Authors: Hongyuan Mao; Scott A Hart; Amy Schink; Brian A Pollok
Journal: J Am Chem Soc Date: 2004-03-10 Impact factor: 15.419

10. Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice.

Authors: Ernie de Boer; Patrick Rodriguez; Edgar Bonte; Jeroen Krijgsveld; Eleni Katsantoni; Albert Heck; Frank Grosveld; John Strouboulis
Journal: Proc Natl Acad Sci U S A Date: 2003-06-11 Impact factor: 11.205

16 in total

1. A noncanonical function of sortase enables site-specific conjugation of small molecules to lysine residues in proteins.

Authors: Joseph J Bellucci; Jayanta Bhattacharyya; Ashutosh Chilkoti
Journal: Angew Chem Int Ed Engl Date: 2014-10-31 Impact factor: 15.336

2. Enrichment of high affinity subclasses and glycoforms from serum-derived IgG using FcγRs as affinity ligands.

Authors: Austin W Boesch; James H Kappel; Alison E Mahan; Thach H Chu; Andrew R Crowley; Nana Y Osei-Owusu; Galit Alter; Margaret E Ackerman
Journal: Biotechnol Bioeng Date: 2018-02-01 Impact factor: 4.530

3. Niclosamide-conjugated polypeptide nanoparticles inhibit Wnt signaling and colon cancer growth.

Authors: Jayanta Bhattacharyya; Xiu-Rong Ren; Robert A Mook; Jiangbo Wang; Ivan Spasojevic; Richard T Premont; Xinghai Li; Ashutosh Chilkoti; Wei Chen
Journal: Nanoscale Date: 2017-08-31 Impact factor: 7.790

4. Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein.

Authors: Yizhi Qi; Miriam Amiram; Weiping Gao; Dewey G McCafferty; Ashutosh Chilkoti
Journal: Macromol Rapid Commun Date: 2013-07-09 Impact factor: 5.734

Review 5. Engineering structure and function using thermoresponsive biopolymers.

Authors: Martha K Pastuszka; J Andrew MacKay
Journal: Wiley Interdiscip Rev Nanomed Nanobiotechnol Date: 2015-06-26

6. Fusion of fibroblast growth factor 21 to a thermally responsive biopolymer forms an injectable depot with sustained anti-diabetic action.

Authors: Caslin A Gilroy; Stefan Roberts; Ashutosh Chilkoti
Journal: J Control Release Date: 2018-03-15 Impact factor: 9.776

10. Non-chromatographic purification of recombinant elastin-like polypeptides and their fusions with peptides and proteins from Escherichia coli.

Authors: Sarah R MacEwan; Wafa Hassouneh; Ashutosh Chilkoti
Journal: J Vis Exp Date: 2014-06-09 Impact factor: 1.355

Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides.