Literature DB >> 18463208

Quadruplex PCR for simultaneous detection of serotype, biotype, toxigenic potential, and central regulating factor of Vibrio cholerae.

Hemant Kumar Khuntia1, Bibhuti Bhusan Pal, Guru Prasada Chhotray.   

Abstract

A quadruplex PCR was developed for the simultaneous detection of genes specific for Vibrio cholerae O1 and/or O139 serogroup (wbe and/or wbf), cholera toxin A subunit (ctxA), toxin-coregulated pilus (tcpA), and central regulating protein ToxR (toxR) in a single tube reaction. This is a simple, rapid, and accurate approach for the detection of toxigenic V. cholerae O1 and/or O139 and can prevent the rapid spread of the disease by early detection.

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Year:  2008        PMID： 18463208      PMCID： PMC2446892          DOI： 10.1128/JCM.00024-08

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Development of a hexaplex PCR assay for rapid detection of virulence and regulatory genes in Vibrio cholerae and Vibrio mimicus.

Authors:  D V Singh; Sree Renjini Isac; R R Colwell
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

2.  Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139.

Authors:  K Hoshino; S Yamasaki; A K Mukhopadhyay; S Chakraborty; A Basu; S K Bhattacharya; G B Nair; T Shimada; Y Takeda
Journal:  FEMS Immunol Med Microbiol       Date:  1998-03

3.  Cholera toxin transcriptional activator toxR is a transmembrane DNA binding protein.

Authors:  V L Miller; R K Taylor; J J Mekalanos
Journal:  Cell       Date:  1987-01-30       Impact factor: 41.582

4.  Incidence and molecular analysis of Vibrio cholerae associated with cholera outbreak subsequent to the super cyclone in Orissa, India.

Authors:  G P Chhotray; B B Pal; H K Khuntia; N R Chowdhury; S Chakraborty; S Yamasaki; T Ramamurthy; Y Takeda; S K Bhattacharya; G Balakrish Nair
Journal:  Epidemiol Infect       Date:  2002-04       Impact factor: 2.451

Review 5.  Cholera.

Authors:  J B Kaper; J G Morris; M M Levine
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

6.  Large outbreak of clinical cholera due to Vibrio cholerae non-O1 in Bangladesh.

Authors:  M J Albert; A K Siddique; M S Islam; A S Faruque; M Ansaruzzaman; S M Faruque; R B Sack
Journal:  Lancet       Date:  1993-03-13       Impact factor: 79.321

7.  Genotypes associated with virulence in environmental isolates of Vibrio cholerae.

Authors:  I N Rivera; J Chun; A Huq; R B Sack; R R Colwell
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

8.  [Distribution of serogroups of Vibrio cholerae non-O1 non-O139 with specific reference to their ability to produce cholera toxin, and addition of novel serogroups].

Authors:  S Yamai; T Okitsu; T Shimada; Y Katsube
Journal:  Kansenshogaku Zasshi       Date:  1997-10

Review 9.  Non-O group 1 Vibrio cholerae: a look at the epidemiology of an occasional pathogen.

Authors:  J G Morris
Journal:  Epidemiol Rev       Date:  1990       Impact factor: 6.222

10.  Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae.

Authors:  H Shirai; M Nishibuchi; T Ramamurthy; S K Bhattacharya; S C Pal; Y Takeda
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948
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  9 in total

1.  RNA colony blot hybridization method for enumeration of culturable Vibrio cholerae and Vibrio mimicus bacteria.

Authors:  Christopher J Grim; Young-Gun Zo; Nur A Hasan; Afsar Ali; Wasimul B Chowdhury; Atiqul Islam; Mohammed H Rashid; Munirul Alam; J Glenn Morris; Anwar Huq; Rita R Colwell
Journal:  Appl Environ Microbiol       Date:  2009-06-26       Impact factor: 4.792

2.  Quadruplex real-time PCR assay for detection and identification of Vibrio cholerae O1 and O139 strains and determination of their toxigenic potential.

Authors:  Jianwei Huang; Yumei Zhu; Huixin Wen; Jiafeng Zhang; Shijie Huang; Jianjun Niu; Qingge Li
Journal:  Appl Environ Microbiol       Date:  2009-09-18       Impact factor: 4.792

3.  Detection of ctx gene positive non-O1/non-O139 V. cholerae in shrimp aquaculture environments.

Authors:  Rao B Madhusudana; P K Surendran
Journal:  J Food Sci Technol       Date:  2011-04-15       Impact factor: 2.701

4.  Rapid spread of Vibrio cholerae O1 El Tor variant in Odisha, Eastern India, in 2008 and 2009.

Authors:  H K Khuntia; B B Pal; S K Samal; S K Kar
Journal:  J Clin Microbiol       Date:  2013-03-20       Impact factor: 5.948

5.  Unique Clones of Vibrio cholerae O1 El Tor with Haitian Type ctxB Allele Implicated in the Recent Cholera Epidemics from Nigeria, Africa.

Authors:  Akinsinde Kehinde Adewale; Gururaja Perumal Pazhani; Iwalokun Bamidele Abiodun; Oluwadun Afolabi; Olukoya Daniel Kolawole; Asish K Mukhopadhyay; Thanadarayan Ramamurthy
Journal:  PLoS One       Date:  2016-08-01       Impact factor: 3.240

6.  Prevalence and molecular characterization of Vibrio cholerae from fruits and salad vegetables sold in Jakarta, Indonesia, using most probable number and PCR.

Authors:  Andrea Budiman; Kevin Kurnia; Diana E Waturangi
Journal:  BMC Res Notes       Date:  2022-03-03

7.  Large outbreak of cholera caused by El Tor variant Vibrio cholerae O1 in the eastern coast of Odisha, India during 2009.

Authors:  B B Pal; H K Khuntia; S K Samal; A S Kerketta; S K Kar; M Karmakar; B Pattnaik
Journal:  Epidemiol Infect       Date:  2013-03-06       Impact factor: 4.434

8.  Waterfowl: the missing link in epidemic and pandemic cholera dissemination?

Authors:  Malka Halpern; Yigal Senderovich; Ido Izhaki
Journal:  PLoS Pathog       Date:  2008-10-31       Impact factor: 6.823

9.  Simultaneous Identification of Ten Bacterial Pathogens Using the Multiplex Ligation Reaction Based on the Probe Melting Curve Analysis.

Authors:  Yixiang Jiang; Lianhua He; Pingfang Wu; Xiaolu Shi; Min Jiang; Yinghui Li; Yiman Lin; Yaqun Qiu; Fang Bai; Yiqun Liao; Qingge Li; RenLi Zhang; Qinghua Hu
Journal:  Sci Rep       Date:  2017-07-19       Impact factor: 4.379
  9 in total

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