Literature DB >> 12409420

Development of a hexaplex PCR assay for rapid detection of virulence and regulatory genes in Vibrio cholerae and Vibrio mimicus.

D V Singh1, Sree Renjini Isac, R R Colwell.   

Abstract

We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in Vibrio cholerae and Vibrio mimicus. This hexaplex PCR proved successful in screening pathogenic-toxigenic and nonpathogenic-nontoxigenic V. cholerae and V. mimicus strains from both clinical and environmental sources.

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Year:  2002        PMID： 12409420      PMCID： PMC139685          DOI： 10.1128/JCM.40.11.4321-4324.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  30 in total

1.  A region of the transmembrane regulatory protein ToxR that tethers the transcriptional activation domain to the cytoplasmic membrane displays wide divergence among Vibrio species.

Authors:  C R Osorio; K E Klose
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

2.  Detection of genes encoding cholera toxin (CT), zonula occludens toxin (ZOT), accessory cholera enterotoxin (ACE) and heat-stable enterotoxin (ST) in Vibrio mimicus clinical strains.

Authors:  L Shi; S Miyoshi; M Hiura; K Tomochika; T Shimada; S Shinoda
Journal:  Microbiol Immunol       Date:  1998       Impact factor: 1.955

3.  Molecular analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 strains: clonal relationships between clinical and environmental isolates.

Authors:  D V Singh; M H Matte; G R Matte; S Jiang; F Sabeena; B N Shukla; S C Sanyal; A Huq; R R Colwell
Journal:  Appl Environ Microbiol       Date:  2001-02       Impact factor: 4.792

4.  Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139.

Authors:  K Hoshino; S Yamasaki; A K Mukhopadhyay; S Chakraborty; A Basu; S K Bhattacharya; G B Nair; T Shimada; Y Takeda
Journal:  FEMS Immunol Med Microbiol       Date:  1998-03

5.  Characterization of the role of the ToxR-modulated outer membrane porins OmpU and OmpT in Vibrio cholerae virulence.

Authors:  D Provenzano; C M Lauriano; K E Klose
Journal:  J Bacteriol       Date:  2001-06       Impact factor: 3.490

6.  Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR.

Authors:  M J Albert; D Islam; S Nahar; F Qadri; S Falklind; A Weintraub
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

7.  Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene.

Authors:  Y B Kim; J Okuda; C Matsumoto; N Takahashi; S Hashimoto; M Nishibuchi
Journal:  J Clin Microbiol       Date:  1999-04       Impact factor: 5.948

8.  Rapid method for species-specific identification of Vibrio cholerae using primers targeted to the gene of outer membrane protein OmpW.

Authors:  B Nandi; R K Nandy; S Mukhopadhyay; G B Nair; T Shimada; A C Ghose
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

9.  Altered expression of the ToxR-regulated porins OmpU and OmpT diminishes Vibrio cholerae bile resistance, virulence factor expression, and intestinal colonization.

Authors:  D Provenzano; K E Klose
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-29       Impact factor: 11.205

Review 10.  Epidemiology, genetics, and ecology of toxigenic Vibrio cholerae.

Authors:  S M Faruque; M J Albert; J J Mekalanos
Journal:  Microbiol Mol Biol Rev       Date:  1998-12       Impact factor: 11.056
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  29 in total

1.  A cholera outbreak of the Vibrio cholerae O1 El Tor variant carrying classical CtxB in northeastern Thailand in 2007.

Authors:  Kazuhisa Okada; Siriporn Chantaroj; Amonrattana Roobthaisong; Shigeyuki Hamada; Pathom Sawanpanyalert
Journal:  Am J Trop Med Hyg       Date:  2010-05       Impact factor: 2.345

2.  A novel triplex quantitative PCR strategy for quantification of toxigenic and nontoxigenic Vibrio cholerae in aquatic environments.

Authors:  Rupert Bliem; Sonja Schauer; Helga Plicka; Adelheid Obwaller; Regina Sommer; Adolf Steinrigl; Munirul Alam; Georg H Reischer; Andreas H Farnleitner; Alexander Kirschner
Journal:  Appl Environ Microbiol       Date:  2015-02-27       Impact factor: 4.792

3.  Virulence profile and clonal relationship among the Vibrio cholerae isolates from ground and surface water in a cholera endemic area during rainy season.

Authors:  A K Goel; M Jain; P Kumar; D V Kamboj; L Singh
Journal:  Folia Microbiol (Praha)       Date:  2010-03-25       Impact factor: 2.099

4.  Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae.

Authors:  Aneta J Gubala; David F Proll
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792

5.  Detection of Vibrio cholerae by real-time nucleic acid sequence-based amplification.

Authors:  Else M Fykse; Gunnar Skogan; William Davies; Jaran Strand Olsen; Janet M Blatny
Journal:  Appl Environ Microbiol       Date:  2007-01-12       Impact factor: 4.792

6.  An incidence of large foodborne outbreak associated with Vibrio mimicus.

Authors:  T Chitov; P Kirikaew; P Yungyune; N Ruengprapan; K Sontikun
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-10-17       Impact factor: 3.267

7.  Novel multiple mutations in the topoisomerase gene of Haitian variant Vibrio cholerae O1.

Authors:  M P Divya; K C Sivakumar; K L Sarada Devi; S Remadevi; Sabu Thomas
Journal:  Antimicrob Agents Chemother       Date:  2014-05-27       Impact factor: 5.191

8.  Molecular characterization of Vibrio cholerae O139 bengal isolated from water and the aquatic plant Eichhornia crassipes in the River Ganga, Varanasi, India.

Authors:  R Bhanumathi; F Sabeena; Sree Renjini Isac; B N Shukla; D V Singh
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

9.  Quadruplex real-time PCR assay for detection and identification of Vibrio cholerae O1 and O139 strains and determination of their toxigenic potential.

Authors:  Jianwei Huang; Yumei Zhu; Huixin Wen; Jiafeng Zhang; Shijie Huang; Jianjun Niu; Qingge Li
Journal:  Appl Environ Microbiol       Date:  2009-09-18       Impact factor: 4.792

10.  Direct detection of Vibrio cholerae and ctxA in Peruvian coastal water and plankton by PCR.

Authors:  Erin K Lipp; Irma N G Rivera; Ana I Gil; Eric M Espeland; Nipa Choopun; Valérie R Louis; Estelle Russek-Cohen; Anwar Huq; Rita R Colwell
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792
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