Literature DB >> 18463206

Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples.

Kwok Hung Chan1, Wing Cheong Yam, Chiu Mei Pang, Kit Man Chan, Siu Yan Lam, Kam Fai Lo, Leo L M Poon, J S Malik Peiris.   

Abstract

The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.

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Year:  2008        PMID： 18463206      PMCID： PMC2446927          DOI： 10.1128/JCM.00315-08

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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