OBJECTIVE: To evaluate the correlation of MIB-1 labeling index (LI) obtained by 2 counting methods with histologic grade and investigate interobserver variability between these methods. STUDY DESIGN: A total of 65 meningiomas were analyzed for proliferation with 2 counting methods by 2 pathologists using MIB-1 antibody. In the first method, the most densely staining areas were counted (HL method). In the second method, randomly selected areas were counted (RS method). RESULTS: MIB-1 values correlated well with histologic grade in both methods. As expected, the tumors with recurrence had significantly higher LIs than the nonrecurrent tumors in each method. However, there was a statistically significant difference in the mean MIB-1 values of between the HL and RS methods. When MIB-1 LI was compared between 2 pathologists, perfect agreement in the HL method and substantial agreement in the RS method were achieved. CONCLUSION: Our results showed that values of MIB LIs differ with different counting methods. Nonetheless, both methods showed good correlation with World Health Organization grades. Therefore standardization of 1 counting method is of great importance for determining a reliable and specific cutoff value in assessing the risk of recurrence in meningiomas.
OBJECTIVE: To evaluate the correlation of MIB-1 labeling index (LI) obtained by 2 counting methods with histologic grade and investigate interobserver variability between these methods. STUDY DESIGN: A total of 65 meningiomas were analyzed for proliferation with 2 counting methods by 2 pathologists using MIB-1 antibody. In the first method, the most densely staining areas were counted (HL method). In the second method, randomly selected areas were counted (RS method). RESULTS:MIB-1 values correlated well with histologic grade in both methods. As expected, the tumors with recurrence had significantly higher LIs than the nonrecurrent tumors in each method. However, there was a statistically significant difference in the mean MIB-1 values of between the HL and RS methods. When MIB-1 LI was compared between 2 pathologists, perfect agreement in the HL method and substantial agreement in the RS method were achieved. CONCLUSION: Our results showed that values of MIB LIs differ with different counting methods. Nonetheless, both methods showed good correlation with World Health Organization grades. Therefore standardization of 1 counting method is of great importance for determining a reliable and specific cutoff value in assessing the risk of recurrence in meningiomas.
Authors: Akash J Patel; Ying-Wooi Wan; Rami Al-Ouran; Jean-Pierre Revelli; Maria F Cardenas; Mazen Oneissi; Liu Xi; Ali Jalali; John F Magnotti; Donna M Muzny; HarshaVardhan Doddapaneni; Sherly Sebastian; Kent A Heck; J Clay Goodman; Shankar P Gopinath; Zhandong Liu; Ganesh Rao; Sharon E Plon; Daniel Yoshor; David A Wheeler; Huda Y Zoghbi; Tiemo J Klisch Journal: Proc Natl Acad Sci U S A Date: 2019-10-07 Impact factor: 11.205
Authors: Omaditya Khanna; Anahita Fathi Kazerooni; Christopher J Farrell; Michael P Baldassari; Tyler D Alexander; Michael Karsy; Benjamin A Greenberger; Jose A Garcia; Chiharu Sako; James J Evans; Kevin D Judy; David W Andrews; Adam E Flanders; Ashwini D Sharan; Adam P Dicker; Wenyin Shi; Christos Davatzikos Journal: Neurosurgery Date: 2021-10-13 Impact factor: 5.315
Authors: Munish Puri; Shelley B Hoover; Stephen M Hewitt; Bih-Rong Wei; Hibret Amare Adissu; Charles H C Halsey; Jessica Beck; Charles Bradley; Sarah D Cramer; Amy C Durham; D Glen Esplin; Chad Frank; L Tiffany Lyle; Lawrence D McGill; Melissa D Sánchez; Paula A Schaffer; Ryan P Traslavina; Elizabeth Buza; Howard H Yang; Maxwell P Lee; Jennifer E Dwyer; R Mark Simpson Journal: J Pathol Inform Date: 2019-02-07
Authors: Bih-Rong Wei; Charles H Halsey; Shelley B Hoover; Munish Puri; Howard H Yang; Brandon D Gallas; Maxwell P Lee; Weijie Chen; Amy C Durham; Jennifer E Dwyer; Melissa D Sánchez; Ryan P Traslavina; Chad Frank; Charles Bradley; Lawrence D McGill; D Glen Esplin; Paula A Schaffer; Sarah D Cramer; L Tiffany Lyle; Jessica Beck; Elizabeth Buza; Qi Gong; Stephen M Hewitt; R Mark Simpson Journal: Acad Pathol Date: 2019-07-11