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Literature DB >> 18456857

Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system combining nucleic acid sequence-based amplification and reverse transcription-loop-mediated isothermal amplification assays.

Shinji Fukuda¹, Yukie Sasaki, Masato Seno.

Abstract

We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.

Mesh：

Substances：
DNA Primers
RNA, Viral

Year: 2008 PMID： 18456857 PMCID： PMC2446545 DOI： 10.1128/AEM.00127-08

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

21 in total

1. Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR.

Authors: Tsutomu Kageyama; Shigeyuki Kojima; Michiyo Shinohara; Kazue Uchida; Shuetsu Fukushi; Fuminori B Hoshino; Naokazu Takeda; Kazuhiko Katayama
Journal: J Clin Microbiol Date: 2003-04 Impact factor: 5.948

2. Evaluation of a broadly reactive nucleic acid sequence based amplification assay for the detection of noroviruses in faecal material.

Authors: C Moore; E M Clark; C I Gallimore; S A Corden; J J Gray; D Westmoreland
Journal: J Clin Virol Date: 2004-04 Impact factor: 3.168

3. Comparative evaluation of RT-PCR, nucleic acid sequence-based amplification (NASBA) and real-time RT-PCR for detection of noroviruses in faecal material.

Authors: Alain Houde; Danielle Leblanc; Elyse Poitras; Pierre Ward; Julie Brassard; Carole Simard; Yvon-Louis Trottier
Journal: J Virol Methods Date: 2006-04-17 Impact factor: 2.014

4. Molecular epidemiology of caliciviruses detected in sporadic and outbreak cases of gastroenteritis in France from December 1998 to February 2004.

Authors: F Bon; K Ambert-Balay; H Giraudon; J Kaplon; S Le Guyader; M Pommepuy; A Gallay; V Vaillant; H de Valk; R Chikhi-Brachet; A Flahaut; P Pothier; E Kohli
Journal: J Clin Microbiol Date: 2005-09 Impact factor: 5.948

5. Norovirus contamination found in oysters worldwide.

Authors: Peter K C Cheng; Derek K K Wong; Thomas W H Chung; Wilina W L Lim
Journal: J Med Virol Date: 2005-08 Impact factor: 2.327

6. Rapid detection of norovirus from fecal specimens by real-time reverse transcription-loop-mediated isothermal amplification assay.

Authors: Shinji Fukuda; Shinichi Takao; Masaru Kuwayama; Yukie Shimazu; Kazuo Miyazaki
Journal: J Clin Microbiol Date: 2006-04 Impact factor: 5.948

7. Coexistence of multiple genotypes, including newly identified genotypes, in outbreaks of gastroenteritis due to Norovirus in Japan.

Authors: Tsutomu Kageyama; Michiyo Shinohara; Kazue Uchida; Shuetsu Fukushi; Fuminori B Hoshino; Shigeyuki Kojima; Reiko Takai; Tomoichiro Oka; Naokazu Takeda; Kazuhiko Katayama
Journal: J Clin Microbiol Date: 2004-07 Impact factor: 5.948

8. Detection of noroviruses in shellfish in the Netherlands.

Authors: Ingeborg L A Boxman; Jeroen J H C Tilburg; Nathalie A J M Te Loeke; Harry Vennema; Klaas Jonker; Enne de Boer; Marion Koopmans
Journal: Int J Food Microbiol Date: 2006-02-24 Impact factor: 5.277

9. Norovirus and foodborne disease, United States, 1991-2000.

Authors: Marc-Alain Widdowson; Alana Sulka; Sandra N Bulens; R Suzanne Beard; Sandra S Chaves; Roberta Hammond; Ellen D P Salehi; Ellen Swanson; Jessica Totaro; Ray Woron; Paul S Mead; Joseph S Bresee; Stephan S Monroe; Roger I Glass
Journal: Emerg Infect Dis Date: 2005-01 Impact factor: 6.883