Literature DB >> 18448621

Evaluation of a multicolor, single-tube technique to enumerate lymphocyte subpopulations.

F Colombo1, A Cattaneo, R Lopa, P Portararo, P Rebulla, L Porretti.   

Abstract

To evaluate the fully automated FACSCanto software, we compared lymphocyte subpopulation counts obtained using three-color FACSCalibur-CELLQuest and six-color FACSCanto-FACSCanto software techniques. High correlations were observed between data obtained with these techniques. Our study indicated that FACSCanto clinical software is accurate and sensitive in single-platform lymphocyte immunophenotyping.

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Year:  2008        PMID: 18448621      PMCID: PMC2446632          DOI: 10.1128/CVI.00481-07

Source DB:  PubMed          Journal:  Clin Vaccine Immunol        ISSN: 1556-679X


  8 in total

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Journal:  Clin Diagn Lab Immunol       Date:  2000-05

Review 2.  Monitoring patients with HIV disease.

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4.  Enumeration of peripheral lymphocyte subsets using 6 vs. 4 color staining: a clinical evaluation of a new flowcytometer.

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Journal:  Cytometry B Clin Cytom       Date:  2006-01       Impact factor: 3.058

5.  Influence of 4- and 6-color flow cytometers and acquisition/analysis softwares on the determination of lymphocyte subsets in HIV infection.

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6.  Evaluation of a dual-color flow cytometry immunophenotyping panel in a multicenter quality assurance program.

Authors:  E L Schenker; L E Hultin; K D Bauer; J Ferbas; J B Margolick; J V Giorgi
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Authors:  F Lacombe; F Durrieu; A Briais; P Dumain; F Belloc; E Bascans; J Reiffers; M R Boisseau; P Bernard
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8.  Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes.

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Journal:  Cytometry       Date:  2002-04-15
  8 in total
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  2 in total

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