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Literature DB >> 18446411

A nonribosomal peptide synthetase gene tzw1 is involved in zwittermicin A biosynthesis in Bacillus thuringiensis G03.

Tiemei Shao¹, Linquan Bai, Jie Zhang, Guangjun Wang, Daqun Liu, Zhuofu Li, Jianmi Liu, Fuping Song, Dafang Huang.

Abstract

A 4.20-kb SspI fragment from Bacillus thuringiensis G03 was cloned and sequenced. Sequencing analysis revealed two complete open reading frames (ORF; tzw1 and tzw2), and one incomplete ORF (tzw3) (GenBank accession no. EU293887). Tzw1 encodes a putative nonribosomal peptide synthetase with thiolation and condensation domains localized at the C-termini, whereas tzw2 and tzw3 encode acyl carrier protein and Acyl-CoA dehydrogenase, respectively. To investigate the function of tzw1 in zwittermicin A (ZA) biosynthesis, an in-frame deletion of 1,461 bp within tzw1 was constructed. The mutant abolished ZA production. Complementation of the mutant with cloned tzw1 restored ZA productivity. These results revealed that tzw1 is required for ZA biosynthesis in B. thuringiensis G03.

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Year: 2008 PMID： 18446411 DOI： 10.1007/s00284-008-9153-5

Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188

12 in total

1. Identification of three Zwittermicin A biosynthesis-related genes from Bacillus thuringiensis subsp. kurstaki strain YBT-1520.

Authors: Changming Zhao; Yi Luo; Chunxu Song; Zhongxin Liu; Shouwen Chen; Ziniu Yu; Ming Sun
Journal: Arch Microbiol Date: 2007-01-16 Impact factor: 2.552

2. Deletion by in vivo recombination shows that the 28-kilodalton cytolytic polypeptide from Bacillus thuringiensis subsp. israelensis is not essential for mosquitocidal activity.

Authors: A Delécluse; J F Charles; A Klier; G Rapoport
Journal: J Bacteriol Date: 1991-06 Impact factor: 3.490

3. Zwittermicin A resistance gene from Bacillus cereus.

Authors: J L Milner; E A Stohl; J Handelsman
Journal: J Bacteriol Date: 1996-07 Impact factor: 3.490

4. Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests.

Authors: Guangjun Wang; Jie Zhang; Fuping Song; Jun Wu; Shuliang Feng; Dafang Huang
Journal: Appl Microbiol Biotechnol Date: 2006-03-28 Impact factor: 4.813

5. Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units.

Authors: Yolande A Chan; Michael T Boyne; Angela M Podevels; Amy K Klimowicz; Jo Handelsman; Neil L Kelleher; Michael G Thomas
Journal: Proc Natl Acad Sci U S A Date: 2006-09-18 Impact factor: 11.205

A nonribosomal peptide synthetase gene tzw1 is involved in zwittermicin A biosynthesis in Bacillus thuringiensis G03.

1. Identification of three Zwittermicin A biosynthesis-related genes from Bacillus thuringiensis subsp. kurstaki strain YBT-1520.

2. Deletion by in vivo recombination shows that the 28-kilodalton cytolytic polypeptide from Bacillus thuringiensis subsp. israelensis is not essential for mosquitocidal activity.

3. Zwittermicin A resistance gene from Bacillus cereus.

4. Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests.

5. Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units.

6. Zwittermicin A biosynthetic cluster.

7. ZmaR, a novel and widespread antibiotic resistance determinant that acetylates zwittermicin A.

8. Transformation and expression of a cloned delta-endotoxin gene in Bacillus thuringiensis.

Review 9. Bacillus thuringiensis and its pesticidal crystal proteins.

10. Target range of zwittermicin A, an aminopolyol antibiotic from Bacillus cereus.

1. Characterization of the complete zwittermicin A biosynthesis gene cluster from Bacillus cereus.

2. Complete genome sequence of Bacillus thuringiensis strain HD521.