Literature DB >> 184435

"Fingerprinting" high molecular weight RNA by two-dimensional gel electrophoresis: application to poliovirus RNA.

Y F Lee, E Wimmer.   

Abstract

Conditions are described under which complete RNase T1 digests of high molecular weight RNA can be separated into numerous components by two-dimensional gel electrophoresis. Small and large oligonucleotides (n = 1 - 2c0) can be resolved without losses. The procedure yields fingerprints which are diagnostic for a particular species of RNA and an index of its purity as will be shown for the genomes of poliovirus type 1 and 2.

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Year:  1976        PMID： 184435      PMCID： PMC343025          DOI： 10.1093/nar/3.7.1647

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Sequence studies of poliovirus RNA. III. Polyuridylic acid and polyadenylic acid as components of the purified poliovirus replicative intermediate.

Authors:  Y Yogo; E Wimmer
Journal:  J Mol Biol       Date:  1975-03-05       Impact factor: 5.469

2.  A physical map of the Rous sarcoma virus genome.

Authors:  J M Coffin; M A Billeter
Journal:  J Mol Biol       Date:  1976-01-25       Impact factor: 5.469

3.  The use of sodium dodecyl sulfate in studies on the interaction of poliovirus and HeLa cells.

Authors:  B MANDEL
Journal:  Virology       Date:  1962-06       Impact factor: 3.616

4.  Sequence studies of poliovirus RNA. I. Characterization of the 5'-terminus.

Authors:  E Wimmer
Journal:  J Mol Biol       Date:  1972-07-28       Impact factor: 5.469

5.  Adenylic acid-rich sequence in RNAs of Rous sarcoma virus and Rauscher mouse leukaemia virus.

Authors:  M M Lai; P H Duesberg
Journal:  Nature       Date:  1972-02-18       Impact factor: 49.962

6.  Purification of the Sabin strain of poliovirus type I through treatment with sarkozyl.

Authors:  M Fiszman; D Bucchini; M Girard
Journal:  J Virol       Date:  1971-05       Impact factor: 5.103

7.  Defective interfering particles of poliovirus. I. Isolation and physical properties.

Authors:  C N Cole; D Smoler; E Wimmer; D Baltimore
Journal:  J Virol       Date:  1971-04       Impact factor: 5.103

8.  Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded.

Authors:  K Dorsch-Häsler; Y Yogo; E Wimmer
Journal:  J Virol       Date:  1975-12       Impact factor: 5.103

9.  The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.

Authors:  A Nomoto; Y F Lee; E Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

10.  Polyadenylic acid at the 3'-terminus of poliovirus RNA.

Authors:  Y Yogo; E Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205
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  21 in total

Review 1.  Methods for subtyping and molecular comparison of human viral genomes.

Authors:  M Arens
Journal:  Clin Microbiol Rev       Date:  1999-10       Impact factor: 26.132

2.  Sequences of large T1 ribonuclease-resistant oligoribonucleotides from protamine mRNA: the overall architecture of protamine mRNA.

Authors:  P L Davies; G H Dixon; A Simoncsits; G C Brownlee
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

3.  Transformation-defective mutants of Rous sarcoma virus with longer sizes of genome RNA and their highly frequent occurrences.

Authors:  M Yoshida; M Yamashita; A Nomoto
Journal:  J Virol       Date:  1979-05       Impact factor: 5.103

4.  Identification of specific fragments containing the 5' end of poliovirus RNA after ribonuclease III digestion.

Authors:  T J Harris; J J Dunn; E Wimmer
Journal:  Nucleic Acids Res       Date:  1978-11       Impact factor: 16.971

5.  Identification of a protein linked to nascent poliovirus RNA and to the polyuridylic acid of negative-strand RNA.

Authors:  R F Pettersson; V Ambros; D Baltimore
Journal:  J Virol       Date:  1978-08       Impact factor: 5.103

6.  Genetic Analysis of Orbiviruses by Using RNase T(1) Oligonucleotide Fingerprints.

Authors:  P J Walker; J N Mansbridge; B M Gorman
Journal:  J Virol       Date:  1980-06       Impact factor: 5.103

7.  The nucleotide sequence of 3C proteinase region of the coxsackievirus A24 variant: comparison of the isolates in Taiwan in 1985-1988.

Authors:  K H Lin; N Takeda; K Miyamura; S Yamazaki; C W Chen
Journal:  Virus Genes       Date:  1991-04       Impact factor: 2.332

8.  Occurrence of partial deletion and substitution of the src gene in the RNA genome of avian sarcoma virus.

Authors:  M M Lai; S S Hu; P K Vogt
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

9.  The 5'-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg.

Authors:  A Nomoto; N Kitamura; F Golini; E Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Initiation of poliovirus plus-strand RNA synthesis in a membrane complex of infected HeLa cells.

Authors:  N Takeda; R J Kuhn; C F Yang; T Takegami; E Wimmer
Journal:  J Virol       Date:  1986-10       Impact factor: 5.103
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