PURPOSE: To investigate the long-term longitudinal profile of retinal ganglion cell (RGC) damage after optic nerve crush with a new technique for in vivo imaging of RGCs. METHODS: A blue-light confocal scanning laser ophthalmoscope (bCSLO; 460 nm excitation, 490 nm detection) was used to image Thy-1 CFP mice aged 6 to 9 months (n = 5) before optic nerve crush, weekly after crush for 3 weeks, and at weeks 10 and 50 after optic nerve crush. A sham procedure was performed in the contralateral eye, and it was imaged as a control. Corresponding retinal areas before and after optic nerve crush were compared, and the fluorescent spots were counted manually. The longitudinal profile of RGC degeneration was modeled and compared with one-phase and two-phase exponential decay equations. RESULTS: A significant and progressive loss of fluorescent spots was found after optic nerve crush with 18.6% +/- 2.3%, 11.3% +/- 3.4%, 8.8% +/- 5.3%, 4.2% +/- 3.1%, and 3.3% +/- 2.1% of Thy-1-expressing RGCs remaining at weeks 1, 2, 3, 10, and 50, respectively, after optic nerve crush (P < 0.001; n = 5). There was no change in the fluorescence density in the contralateral control (P = 0.893). Two-phase exponential decay (y = 0.03 + 0.83e(-)(2.78t) + 0.14e(-)(0.30t)) was a better fit than one-phase exponential decay (y = 0.94e(-)(1.93t) + 0.06; P = 0.003) equations, with half-lives of fast phase and slow phase of 1.7 days and 16.3 days, respectively. CONCLUSIONS: The longitudinal profile of RGC degeneration after optic nerve crush is characterized by a two-phase exponential decay model. bCSLO imaging provides an efficient and noninvasive approach to the longitudinal study of progressive RGC damage.
PURPOSE: To investigate the long-term longitudinal profile of retinal ganglion cell (RGC) damage after optic nerve crush with a new technique for in vivo imaging of RGCs. METHODS: A blue-light confocal scanning laser ophthalmoscope (bCSLO; 460 nm excitation, 490 nm detection) was used to image Thy-1CFPmice aged 6 to 9 months (n = 5) before optic nerve crush, weekly after crush for 3 weeks, and at weeks 10 and 50 after optic nerve crush. A sham procedure was performed in the contralateral eye, and it was imaged as a control. Corresponding retinal areas before and after optic nerve crush were compared, and the fluorescent spots were counted manually. The longitudinal profile of RGC degeneration was modeled and compared with one-phase and two-phase exponential decay equations. RESULTS: A significant and progressive loss of fluorescent spots was found after optic nerve crush with 18.6% +/- 2.3%, 11.3% +/- 3.4%, 8.8% +/- 5.3%, 4.2% +/- 3.1%, and 3.3% +/- 2.1% of Thy-1-expressing RGCs remaining at weeks 1, 2, 3, 10, and 50, respectively, after optic nerve crush (P < 0.001; n = 5). There was no change in the fluorescence density in the contralateral control (P = 0.893). Two-phase exponential decay (y = 0.03 + 0.83e(-)(2.78t) + 0.14e(-)(0.30t)) was a better fit than one-phase exponential decay (y = 0.94e(-)(1.93t) + 0.06; P = 0.003) equations, with half-lives of fast phase and slow phase of 1.7 days and 16.3 days, respectively. CONCLUSIONS: The longitudinal profile of RGC degeneration after optic nerve crush is characterized by a two-phase exponential decay model. bCSLO imaging provides an efficient and noninvasive approach to the longitudinal study of progressive RGC damage.
Authors: Glyn Chidlow; Robert Casson; Paloma Sobrado-Calvo; Manuel Vidal-Sanz; Neville N Osborne Journal: Mol Vis Date: 2005-06-02 Impact factor: 2.367
Authors: Christopher K S Leung; James D Lindsey; Jonathan G Crowston; Won-Kyu Ju; Qwan Liu; Dirk-Uwe Bartsch; Robert N Weinreb Journal: J Neurosci Methods Date: 2007-11-07 Impact factor: 2.390
Authors: M Francesca Cordeiro; Li Guo; Vy Luong; Glen Harding; Wei Wang; Helen E Jones; Stephen E Moss; Adam M Sillito; Frederick W Fitzke Journal: Proc Natl Acad Sci U S A Date: 2004-08-30 Impact factor: 11.205
Authors: Farid Ahmed; Kevin M Brown; Dietrich A Stephan; John C Morrison; Elaine C Johnson; Stanislav I Tomarev Journal: Invest Ophthalmol Vis Sci Date: 2004-04 Impact factor: 4.799
Authors: Kevin C Chan; Yu Yu; Shuk Han Ng; Heather K Mak; Yolanda W Y Yip; Yolandi van der Merwe; Tianmin Ren; Jasmine S Y Yung; Sayantan Biswas; Xu Cao; Ying Chau; Christopher K S Leung Journal: Acta Biomater Date: 2019-06-06 Impact factor: 8.947
Authors: Gustavo C Munguba; Sanja Galeb; Yuan Liu; David C Landy; Daisy Lam; Andrew Camp; Sinthia Samad; Mary L Tapia; Richard K Lee Journal: Invest Ophthalmol Vis Sci Date: 2014-09-16 Impact factor: 4.799
Authors: G B Whitworth; B C Misaghi; D M Rosenthal; E A Mills; D J Heinen; A H Watson; C W Ives; S H Ali; K Bezold; N Marsh-Armstrong; F L Watson Journal: Dev Biol Date: 2016-07-26 Impact factor: 3.582