PURPOSE: We report a noninvasive method for the monitoring of retinal ganglion cell (RGC) survival in live mice utilizing standard fluorescence microscopy. PROCEDURES: Transgenic mice expressing cyan fluorescent protein (CFP) under the regulation of an RGC-specific promoter Thy1 were used in this study. RESULTS: We established that Thy1-CFP expression is a quantitative reflection of the number of surviving RGCs, the fluorescence emission is stable for at least a year and that the loss of fluorescence correlates directly to glaucomatous damage. In high pressure glaucoma model, the peripheral retina is preferentially affected. CONCLUSIONS: Our live-imaging technique allows for the longitudinal assessment of RGC survival from the same animal. Noninvasive monitoring of neuronal cell death and survival is a powerful technique that would allow investigators to validate new potential glaucoma therapy based on neuroprotection.
PURPOSE: We report a noninvasive method for the monitoring of retinal ganglion cell (RGC) survival in live mice utilizing standard fluorescence microscopy. PROCEDURES: Transgenic mice expressing cyan fluorescent protein (CFP) under the regulation of an RGC-specific promoter Thy1 were used in this study. RESULTS: We established that Thy1-CFP expression is a quantitative reflection of the number of surviving RGCs, the fluorescence emission is stable for at least a year and that the loss of fluorescence correlates directly to glaucomatous damage. In high pressure glaucoma model, the peripheral retina is preferentially affected. CONCLUSIONS: Our live-imaging technique allows for the longitudinal assessment of RGC survival from the same animal. Noninvasive monitoring of neuronal cell death and survival is a powerful technique that would allow investigators to validate new potential glaucoma therapy based on neuroprotection.
Authors: David S Friedman; Roger C W Wolfs; Benita J O'Colmain; Barbara E Klein; Hugh R Taylor; Shelia West; M Cristina Leske; Paul Mitchell; Nathan Congdon; John Kempen Journal: Arch Ophthalmol Date: 2004-04
Authors: Daniel C Gray; William Merigan; Jessica I Wolfing; Bernard P Gee; Jason Porter; Alfredo Dubra; Ted H Twietmeyer; Kamran Ahamd; Remy Tumbar; Fred Reinholz; David R Williams Journal: Opt Express Date: 2006-08-07 Impact factor: 3.894
Authors: M Francesca Cordeiro; Li Guo; Vy Luong; Glen Harding; Wei Wang; Helen E Jones; Stephen E Moss; Adam M Sillito; Frederick W Fitzke Journal: Proc Natl Acad Sci U S A Date: 2004-08-30 Impact factor: 11.205
Authors: Stephen H Tsang; Lawrence Chan; Yi-Ting Tsai; Wen-Hsuan Wu; Chun-Wei Hsu; Jin Yang; Joaquin Tosi; Katherine J Wert; Richard J Davis; Vinit B Mahajan Journal: Trans Am Ophthalmol Soc Date: 2014-07
Authors: Michael Yamakawa; Samuel M Santosa; Neeraj Chawla; Evguenia Ivakhnitskaia; Matthew Del Pino; Sebastian Giakas; Arnold Nadel; Sneha Bontu; Arjun Tambe; Kai Guo; Kyu-Yeon Han; Maria Soledad Cortina; Charles Yu; Mark I Rosenblatt; Jin-Hong Chang; Dimitri T Azar Journal: Biochim Biophys Acta Gen Subj Date: 2020-03-12 Impact factor: 3.770