Literature DB >> 18438417

Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system.

Konstantin V Korotkov1, Wim G J Hol.   

Abstract

Gram-negative bacteria translocate various proteins including virulence factors across their outer membrane via type 2 secretion systems (T2SSs). T2SSs are thought to contain a pseudopilus, a subcomplex formed by one major and several minor pseudopilins. We report the crystal structure of the complex formed by three minor pseudopilins from enterotoxigenic Escherichia coli. The GspK-GspI-GspJ complex has quasihelical characteristics and an architecture consistent with a localization at the pseudopilus tip. The alpha-domain of GspK has a previously unobserved fold with an unexpected dinuclear metal binding site. The area surrounding its disulfide bridge is conserved and might interact with other T2SS components or with secreted proteins.

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Year:  2008        PMID： 18438417     DOI： 10.1038/nsmb.1426

Source DB:  PubMed          Journal:  Nat Struct Mol Biol        ISSN： 1545-9985            Impact factor:   15.369


  64 in total

1.  Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation.

Authors:  David A Cisneros; Peter J Bond; Anthony P Pugsley; Manuel Campos; Olivera Francetic
Journal:  EMBO J       Date:  2011-12-09       Impact factor: 11.598

Review 2.  On the path to uncover the bacterial type II secretion system.

Authors:  Badreddine Douzi; Alain Filloux; Romé Voulhoux
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2012-04-19       Impact factor: 6.237

3.  Detailed structural and assembly model of the type II secretion pilus from sparse data.

Authors:  Manuel Campos; Michaël Nilges; David A Cisneros; Olivera Francetic
Journal:  Proc Natl Acad Sci U S A       Date:  2010-07-02       Impact factor: 11.205

4.  Lessons from high-throughput protein crystallization screening: 10 years of practical experience.

Authors:  Joseph R Luft; Edward H Snell; George T Detitta
Journal:  Expert Opin Drug Discov       Date:  2011-03-22       Impact factor: 6.098

Review 5.  Type IV pili in Gram-positive bacteria.

Authors:  Stephen Melville; Lisa Craig
Journal:  Microbiol Mol Biol Rev       Date:  2013-09       Impact factor: 11.056

6.  Expression, purification, crystallization and preliminary X-ray studies of Vibrio cholerae pseudopilin EpsH.

Authors:  Kannan Raghunathan; Frank S Vago; Terry Ball; Nafissa Yakubova; David Grindem; William J Wedemeyer; Dennis N Arvidson
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-06-27

7.  Structure of the minor pseudopilin XcpW from the Pseudomonas aeruginosa type II secretion system.

Authors:  Laura P Franz; Badreddine Douzi; Eric Durand; David H Dyer; Romé Voulhoux; Katrina T Forest
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-01-15

8.  Identification, immunogenicity, and cross-reactivity of type IV pilin and pilin-like proteins from Clostridium difficile.

Authors:  Grace A Maldarelli; Leon De Masi; Erik C von Rosenvinge; Mihaela Carter; Michael S Donnenberg
Journal:  Pathog Dis       Date:  2014-02-18       Impact factor: 3.166

9.  Crystal structure of the full-length ATPase GspE from the Vibrio vulnificus type II secretion system in complex with the cytoplasmic domain of GspL.

Authors:  Connie Lu; Konstantin V Korotkov; Wim G J Hol
Journal:  J Struct Biol       Date:  2014-08-01       Impact factor: 2.867

10.  Structure of the cholera toxin secretion channel in its closed state.

Authors:  Steve L Reichow; Konstantin V Korotkov; Wim G J Hol; Tamir Gonen
Journal:  Nat Struct Mol Biol       Date:  2010-09-19       Impact factor: 15.369
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