INTRODUCTION: The objective of this study was to evaluate the normal cardiovascular and ECG parameters in freely moving minipigs and to use these data as the basis of pharmacological drug safety evaluation. METHODS: 7 Göttingen Minipigs were equipped with radiotelemetry transmitters (ITS). Aortic pressure (AP), left ventricular pressure (LVP), lead II of the ECG and body temperature were continuously monitored. Notocord HEM 4.2 software was used for data acquisition. Power calculations for the various parameters were done to assess appropriate sample sizes. RESULTS: We obtained excellent signal quality and found stable hemodynamic parameters with a low intrinsic heart rate in the Göttingen Minipig. After oral dosing of vehicle, the hemodynamic parameters returned quickly to baseline values indicating that the procedure was well tolerated. The heart rate dependency of the QT interval had to be corrected individually. A sufficient power could be achieved with a sample size of 4 due to the low variability of the parameters measured. DISCUSSION: These are, to our knowledge, the first data documenting the course of systemic arterial and ventricular hemodynamic parameters in the freely moving Göttingen Minipig over 24 h. As such, they may serve as a basis for future studies in which drug effects are studied in these animals.
INTRODUCTION: The objective of this study was to evaluate the normal cardiovascular and ECG parameters in freely moving minipigs and to use these data as the basis of pharmacological drug safety evaluation. METHODS: 7 Göttingen Minipigs were equipped with radiotelemetry transmitters (ITS). Aortic pressure (AP), left ventricular pressure (LVP), lead II of the ECG and body temperature were continuously monitored. Notocord HEM 4.2 software was used for data acquisition. Power calculations for the various parameters were done to assess appropriate sample sizes. RESULTS: We obtained excellent signal quality and found stable hemodynamic parameters with a low intrinsic heart rate in the Göttingen Minipig. After oral dosing of vehicle, the hemodynamic parameters returned quickly to baseline values indicating that the procedure was well tolerated. The heart rate dependency of the QT interval had to be corrected individually. A sufficient power could be achieved with a sample size of 4 due to the low variability of the parameters measured. DISCUSSION: These are, to our knowledge, the first data documenting the course of systemic arterial and ventricular hemodynamic parameters in the freely moving Göttingen Minipig over 24 h. As such, they may serve as a basis for future studies in which drug effects are studied in these animals.
Authors: Trine P Ludvigsen; Niels Wiinberg; Christina J Jensen; Annemette T Callesen; Regitze W Andersen; Anne Sofie H Jørgensen; Berit Ø Christoffersen; Henrik D Pedersen; Sophia G Moesgaard; Lisbeth H Olsen Journal: Comp Med Date: 2014-12 Impact factor: 0.982
Authors: David S Park; Marina Cerrone; Gregory Morley; Carolina Vasquez; Steven Fowler; Nian Liu; Scott A Bernstein; Fang-Yu Liu; Jie Zhang; Christopher S Rogers; Silvia G Priori; Larry A Chinitz; Glenn I Fishman Journal: J Clin Invest Date: 2014-12-15 Impact factor: 14.808
Authors: Mallory K Tate; William S Lawrence; Randy L Gourley; Diana L Zavala; Lori E Weaver; Scott T Moen; Johnny W Peterson Journal: BMC Res Notes Date: 2011-09-05
Authors: Semone B Myrie; Leslie L McKnight; J Christopher King; John J McGuire; Bruce N Van Vliet; Robert F Bertolo Journal: Comp Med Date: 2012-08 Impact factor: 0.982
Authors: Stefan Koudstaal; Sanne Jansen of Lorkeers; Johannes M I H Gho; Gerardus P J van Hout; Marlijn S Jansen; Paul F Gründeman; Gerard Pasterkamp; Pieter A Doevendans; Imo E Hoefer; Steven A J Chamuleau Journal: J Vis Exp Date: 2014-04-25 Impact factor: 1.355
Authors: Christian B Poulsen; Mads Damkjær; Bjørn O Hald; Tobias Wang; Niels-Henrik Holstein-Rathlou; Jens Christian B Jacobsen Journal: Physiol Rep Date: 2016-06