Literature DB >> 18430568

Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology.

V Deepak1, K Kalishwaralal, S Ramkumarpandian, S Venkatesh Babu, S R Senthilkumar, G Sangiliyandi.   

Abstract

Response surface methodology and central composite rotary design (CCRD) was employed to optimize a fermentation medium for the production of Nattokinase by Bacillus subtilis at pH 7.5. The four variables involved in this study were Glucose, Peptone, CaCl2, and MgSO4. The statistical analysis of the results showed that, in the range studied; only peptone had a significant effect on Nattokinase production. The optimized medium containing (%) Glucose: 1, Peptone: 5.5, MgSO4: 0.2 and CaCl2: 0.5 resulted in 2-fold increased level of Nattokinase (3194.25U/ml) production compared to initial level (1599.09U/ml) after 10h of fermentation. Nattokinase production was checked with fibrinolytic activity.

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Year:  2008        PMID： 18430568     DOI： 10.1016/j.biortech.2008.03.018

Source DB:  PubMed          Journal:  Bioresour Technol        ISSN： 0960-8524            Impact factor:   9.642


  25 in total

1.  Optimization of Parameters that Affect the Activity of the Alkaline Protease from Halotolerant Bacterium, Bacillus acquimaris VITP4, by the Application of Response Surface Methodology and Evaluation of the Storage Stability of the Enzyme.

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Review 2.  Microbial production of nattokinase: current progress, challenge and prospect.

Authors:  Dongbo Cai; Chengjun Zhu; Shouwen Chen
Journal:  World J Microbiol Biotechnol       Date:  2017-04-04       Impact factor: 3.312

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Journal:  J Zhejiang Univ Sci B       Date:  2013-04       Impact factor: 3.066

4.  Isolation, identification, optimization, and metabolite profiling of Streptomyces sparsus VSM-30.

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Journal:  3 Biotech       Date:  2017-07-01       Impact factor: 2.406

5.  Optimization of electrochemical dechlorination of trichloroethylene in reducing electrolytes.

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Journal:  Water Res       Date:  2012-01-08       Impact factor: 11.236

6.  Statistical Optimization of the Production of NaCl-Tolerant Proteases by a Moderate Halophile, Virgibacillus sp. SK37.

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7.  Optimization of lipid extraction from Salvinia molesta for biodiesel production using RSM and its FAME analysis.

Authors:  M Mubarak; A Shaija; T V Suchithra
Journal:  Environ Sci Pollut Res Int       Date:  2016-04-05       Impact factor: 4.223

Review 8.  Fibrinolytic Enzymes for Thrombolytic Therapy.

Authors:  Swaroop S Kumar; Abdulhameed Sabu
Journal:  Adv Exp Med Biol       Date:  2019       Impact factor: 2.622

9.  A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design.

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Journal:  Biotechnol Res Int       Date:  2011-05-30

10.  Actinobacillus succinogenes ATCC 55618 fermentation medium optimization for the production of succinic acid by response surface methodology.

Authors:  Li-Wen Zhu; Cheng-Cheng Wang; Rui-Sang Liu; Hong-Mei Li; Duan-Ji Wan; Ya-Jie Tang
Journal:  J Biomed Biotechnol       Date:  2012-10-03
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