Literature DB >> 18429274

Chemical cleavage of proteins in solution.

Dan L Crimmins1, Sheenah M Mische, Nancy D Denslow.   

Abstract

Described in this unit are five basic protocols that are widely used for specific and efficient chemical cleavage of proteins in solution. Cyanogen bromide (CNBr) cleaves at methionine (Met) residues; BNPS-skatole cleaves at tryptophan (Trp) residues; formic acid cleaves at aspartic acid-proline (Asp-Pro) peptide bonds; hydroxylamine cleaves at asparagine-glycine (Asn-Gly) peptide bonds, and 2-nitro-5-thiocyanobenzoic acid (NTCB) cleaves at cysteine (Cys) residues. Because the above loci are at relatively low abundance in most proteins, digestion with these agents will yield relatively long peptides.

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Year:  2005        PMID： 18429274     DOI： 10.1002/0471140864.ps1104s40

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  17 in total

1.  N-terminal protein characterization by mass spectrometry after cyanogen bromide cleavage using combined microscale liquid- and solid-phase derivatization.

Authors:  Heinz Nika; David H Hawke; Ruth Hogue Angeletti
Journal:  J Biomol Tech       Date:  2014-04

2.  Pronase E-Based Generation of Fluorescent Peptide Fragments: Tracking Intracellular Peptide Fate in Single Cells.

Authors:  Emilie R Mainz; Nicholas C Dobes; Nancy L Allbritton
Journal:  Anal Chem       Date:  2015-07-24       Impact factor: 6.986

Review 3.  Optimized approaches for quantification of drug transporters in tissues and cells by MRM proteomics.

Authors:  Bhagwat Prasad; Jashvant D Unadkat
Journal:  AAPS J       Date:  2014-04-22       Impact factor: 4.009

4.  The photosynthetic cytochrome c 550 from the diatom Phaeodactylum tricornutum.

Authors:  Pilar Bernal-Bayard; Leonor Puerto-Galán; Inmaculada Yruela; Inés García-Rubio; Carmen Castell; José M Ortega; Pablo J Alonso; Mercedes Roncel; Jesús I Martínez; Manuel Hervás; José A Navarro
Journal:  Photosynth Res       Date:  2016-12-28       Impact factor: 3.573

5.  Production of a de-novo designed antimicrobial peptide in Nicotiana benthamiana.

Authors:  Benjamin Zeitler; Antonie Bernhard; Helge Meyer; Michael Sattler; Hans-Ulrich Koop; Christian Lindermayr
Journal:  Plant Mol Biol       Date:  2012-12-16       Impact factor: 4.076

Review 6.  Current strategies for protein production and purification enabling membrane protein structural biology.

Authors:  Aditya Pandey; Kyungsoo Shin; Robin E Patterson; Xiang-Qin Liu; Jan K Rainey
Journal:  Biochem Cell Biol       Date:  2016-01-20       Impact factor: 3.626

7.  Seamless bead to microarray screening: rapid identification of the highest affinity protein ligands from large combinatorial libraries.

Authors:  John M Astle; Levi S Simpson; Yong Huang; M Muralidhar Reddy; Rosemary Wilson; Steven Connell; Johnnie Wilson; Thomas Kodadek
Journal:  Chem Biol       Date:  2010-01-29

8.  Biochemical Characterization of AP Lyase and m6A Demethylase Activities of Human AlkB Homologue 1 (ALKBH1).

Authors:  Tina A Müller; Michael A Tobar; Madison N Perian; Robert P Hausinger
Journal:  Biochemistry       Date:  2017-03-21       Impact factor: 3.162

9.  A Tyr residue in the reverse transcriptase domain can mimic the protein-priming Tyr residue in the terminal protein domain of a hepadnavirus P protein.

Authors:  Jürgen Beck; Michael Nassal
Journal:  J Virol       Date:  2011-05-18       Impact factor: 5.103

10.  Recifin A, Initial Example of the Tyr-Lock Peptide Structural Family, Is a Selective Allosteric Inhibitor of Tyrosyl-DNA Phosphodiesterase I.

Authors:  Lauren R H Krumpe; Brice A P Wilson; Christophe Marchand; Suthananda N Sunassee; Alun Bermingham; Wenjie Wang; Edmund Price; Tad Guszczynski; James A Kelley; Kirk R Gustafson; Yves Pommier; K Johan Rosengren; Christina I Schroeder; Barry R O'Keefe
Journal:  J Am Chem Soc       Date:  2020-12-02       Impact factor: 15.419
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