Literature DB >> 18429221

Protein detection in gels using fixation.

Lynn A Echan1, David W Speicher.   

Abstract

This unit has been recently updated to include information on preformulated gel stains as well as new protocols for Sypro Ruby and silver staining, and gel imaging methodology. Other previously published protocols are also provided for both rapid and acid-based Coomassie blue staining, and alternate methods for silver staining (i.e., nonammoniacal silver staining, rapid silver staining, and an enhanced-background, two-stage method).

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Year:  2002        PMID: 18429221      PMCID: PMC5647780          DOI: 10.1002/0471140864.ps1005s29

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN: 1934-3655


  13 in total

1.  An improved formulation of SYPRO Ruby protein gel stain: comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulation.

Authors:  Kiera N Berggren; Birte Schulenberg; Mary F Lopez; Thomas H Steinberg; Alla Bogdanova; Gary Smejkal; Annie Wang; Wayne F Patton
Journal:  Proteomics       Date:  2002-05       Impact factor: 3.984

Review 2.  Digital imaging considerations for gel electrophoresis analysis systems.

Authors:  M D Miller; R A Acey; L Y Lee; A J Edwards
Journal:  Electrophoresis       Date:  2001-03       Impact factor: 3.535

Review 3.  Imaging and detection technologies for image analysis in electrophoresis.

Authors:  K Miura
Journal:  Electrophoresis       Date:  2001-03       Impact factor: 3.535

4.  Postelectrophoretic staining of proteins separated by two-dimensional gel electrophoresis using SYPRO dyes.

Authors:  J X Yan; R A Harry; C Spibey; M J Dunn
Journal:  Electrophoresis       Date:  2000-11       Impact factor: 3.535

Review 5.  Protein stains for proteomic applications: which, when, why?

Authors:  Ingrid Miller; Johanne Crawford; Elisabetta Gianazza
Journal:  Proteomics       Date:  2006-10       Impact factor: 3.984

6.  Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.

Authors:  K Weber; J R Pringle; M Osborn
Journal:  Methods Enzymol       Date:  1972       Impact factor: 1.600

7.  A new staining technique for proteins in polyacrylamide gels using coomassie brilliant blue G250.

Authors:  R W Blakesley; J A Boezi
Journal:  Anal Biochem       Date:  1977-10       Impact factor: 3.365

Review 8.  A thousand points of light: the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics.

Authors:  W F Patton
Journal:  Electrophoresis       Date:  2000-04       Impact factor: 3.535

9.  Coomassie blue as a near-infrared fluorescent stain: a systematic comparison with Sypro Ruby for in-gel protein detection.

Authors:  R Hussain Butt; Jens R Coorssen
Journal:  Mol Cell Proteomics       Date:  2013-09-16       Impact factor: 5.911

Review 10.  Imaging technologies for the detection of multiple stains in proteomics.

Authors:  Kenji Miura
Journal:  Proteomics       Date:  2003-07       Impact factor: 3.984

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  4 in total

1.  Analysis of the mitochondrial proteome in multiple sclerosis cortex.

Authors:  Laurie Broadwater; Ashish Pandit; Robert Clements; Sausan Azzam; Jonathan Vadnal; Michael Sulak; V Wee Yong; Ernest J Freeman; Roger B Gregory; Jennifer McDonough
Journal:  Biochim Biophys Acta       Date:  2011-02-02

2.  Protein tyrosine phosphatase assays.

Authors:  Ulrike Lorenz
Journal:  Curr Protoc Immunol       Date:  2011-04

3.  Yeast Secretes High Amounts of Human Calreticulin without Cellular Stress.

Authors:  Rūta Zinkevičiūtė; Raimundas Ražanskas; Algirdas Kaupinis; Neringa Macijauskaitė; Evaldas Čiplys; Gunnar Houen; Rimantas Slibinskas
Journal:  Curr Issues Mol Biol       Date:  2022-04-19       Impact factor: 2.976

4.  Site-Specific Protein Labeling via Sortase-Mediated Transpeptidation.

Authors:  John M Antos; Jessica Ingram; Tao Fang; Novalia Pishesha; Matthias C Truttmann; Hidde L Ploegh
Journal:  Curr Protoc Protein Sci       Date:  2017-08-01
  4 in total

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