Literature DB >> 1842920

A PCR-enhanced method for determining the 5' end sequence of mRNAs.

M A Hofmann1, D A Brian.   

Abstract

A method was developed to amplify ligated cDNA copies of mRNA 5' ends before cloning and sequencing. The method facilitates the study of the 5' end sequence on many molecules of an individual mRNA species. It can also be used to study the 5' end sequence on other kinds of single-stranded RNA molecules.

Mesh：

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Year:  1991        PMID： 1842920     DOI： 10.1101/gr.1.1.43

Source DB:  PubMed          Journal:  PCR Methods Appl        ISSN： 1054-9803


  10 in total

1.  The nucleocapsid protein gene of bovine coronavirus is bicistronic.

Authors:  S D Senanayake; M A Hofmann; J L Maki; D A Brian
Journal:  J Virol       Date:  1992-09       Impact factor: 5.103

2.  Replication of murine coronavirus defective interfering RNA from negative-strand transcripts.

Authors:  M Joo; S Banerjee; S Makino
Journal:  J Virol       Date:  1996-09       Impact factor: 5.103

3.  The seizure locus encodes the Drosophila homolog of the HERG potassium channel.

Authors:  X J Wang; E R Reynolds; P Déak; L M Hall
Journal:  J Neurosci       Date:  1997-02-01       Impact factor: 6.167

4.  A translation-attenuating intraleader open reading frame is selected on coronavirus mRNAs during persistent infection.

Authors:  M A Hofmann; S D Senanayake; D A Brian
Journal:  Proc Natl Acad Sci U S A       Date:  1993-12-15       Impact factor: 11.205

5.  5' termini of Pichinde arenavirus S RNAs and mRNAs contain nontemplated nucleotides.

Authors:  S J Polyak; S Zheng; D G Harnish
Journal:  J Virol       Date:  1995-05       Impact factor: 5.103

6.  A cis-acting function for the coronavirus leader in defective interfering RNA replication.

Authors:  R Y Chang; M A Hofmann; P B Sethna; D A Brian
Journal:  J Virol       Date:  1994-12       Impact factor: 5.103

7.  The 5' end of coronavirus minus-strand RNAs contains a short poly(U) tract.

Authors:  M A Hofmann; D A Brian
Journal:  J Virol       Date:  1991-11       Impact factor: 5.103

8.  Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein.

Authors:  T L Lewis; H B Greenberg; J E Herrmann; L S Smith; S M Matsui
Journal:  J Virol       Date:  1994-01       Impact factor: 5.103

9.  Cloning, genomic organization, and osmotic response of the aldose reductase gene.

Authors:  J D Ferraris; C K Williams; B M Martin; M B Burg; A García-Pérez
Journal:  Proc Natl Acad Sci U S A       Date:  1994-10-25       Impact factor: 11.205

10.  A conserved sequence motif at the 5' terminus of the Southampton virus genome is characteristic of the Caliciviridae.

Authors:  P R Lambden; B Liu; I N Clarke
Journal:  Virus Genes       Date:  1995       Impact factor: 2.332
  10 in total

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