Literature DB >> 18429085

Quantitation of nucleic acids with absorption spectroscopy.

S Gallagher1.   

Abstract

This appendix describes a protocol for measuring absorption of nucleic acid samples at several different wavelengths to assess purity and concentration. A(260) measurements are quantitative for relatively pure nucleic acid preparations in microgram quantities. Absorbance readings cannot discriminate between DNA and RNA; however, the ratio of A at 260 and 280 nm can be used as an indicator of nucleic acid purity.

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Year:  2001        PMID： 18429085     DOI： 10.1002/0471140864.psa04ks13

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  6 in total

1.  L-methionine-induced alterations in molecular signatures in MCF-7 and LNCaP cancer cells.

Authors:  Maximo A Benavides; Dong Hu; Marie Kristine Baraoidan; Annette Bruno; Pan Du; Simon Lin; Wancai Yang; Kirby I Bland; William E Grizzle; Maarten C Bosland
Journal:  J Cancer Res Clin Oncol       Date:  2010-05-09       Impact factor: 4.553

2.  Suppression by L-methionine of cell cycle progression in LNCaP and MCF-7 cells but not benign cells.

Authors:  Maximo A Benavides; Karen L Hagen; Wenfeng Fang; Pan Du; Simon Lin; Mary P Moyer; Wancai Yang; Kirby I Bland; William E Grizzle; Maarten C Bosland
Journal:  Anticancer Res       Date:  2010-06       Impact factor: 2.480

3.  Culture temperature affects human chondrocyte messenger RNA expression in monolayer and pellet culture systems.

Authors:  Akira Ito; Momoko Nagai; Junichi Tajino; Shoki Yamaguchi; Hirotaka Iijima; Xiangkai Zhang; Tomoki Aoyama; Hiroshi Kuroki
Journal:  PLoS One       Date:  2015-05-26       Impact factor: 3.240

4.  DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis.

Authors:  Gema Lucena-Aguilar; Ana María Sánchez-López; Cristina Barberán-Aceituno; José Antonio Carrillo-Ávila; José Antonio López-Guerrero; Rocío Aguilar-Quesada
Journal:  Biopreserv Biobank       Date:  2016-05-09       Impact factor: 2.300

5.  Quality assessment of RNA in long-term storage: The All Our Families biorepository.

Authors:  Nikki L Stephenson; Kylie K Hornaday; Chelsea T A Doktorchik; Andrew W Lyon; Suzanne C Tough; Donna M Slater
Journal:  PLoS One       Date:  2020-12-01       Impact factor: 3.240

6.  HPV molecular detection from urine versus cervical samples: an alternative for HPV screening in indigenous populations.

Authors:  Francisco I Torres-Rojas; Miguel A Mendoza-Catalán; Luz Del C Alarcón-Romero; Isela Parra-Rojas; Sergio Paredes-Solís; Marco A Leyva-Vázquez; Jair E Cortes-Arciniega; Carlos J Bracamontes-Benítez; Berenice Illades-Aguiar
Journal:  PeerJ       Date:  2021-06-17       Impact factor: 2.984
  6 in total

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