BACKGROUND: Methionine inhibits proliferation of breast and prostate cancer cells. Here, we determined the influence of L-methionine on functional molecular signatures in these cell lines. METHODS: MCF-7 and LNCaP cells were treated with L-methionine (5 mg/ml) for 72 h. Changes in molecular signatures of these cells were examined by microarray analysis of 15,814 probes in triplicate experiments. RESULTS: In LNCaP cells, 325 genes were up-regulated by methionine, and 517 genes down-regulated. In MCF-7 cells, 86 genes were up-regulated and 135 genes down-regulated. Ninety-eight genes were regulated in the same direction by methionine in both cells lines, and five other genes were changed in expression in opposite directions. CONCLUSION: Several of the up-regulated genes encode proteins involved in cellular redox regulation, suggesting that methionine may enhance antioxidant mechanisms. Many of the down-regulated genes belong to protein kinase families that may be related to the anti-proliferative effects of methionine on breast and prostate cancer cells.
BACKGROUND:Methionine inhibits proliferation of breast and prostate cancer cells. Here, we determined the influence of L-methionine on functional molecular signatures in these cell lines. METHODS:MCF-7 and LNCaP cells were treated with L-methionine (5 mg/ml) for 72 h. Changes in molecular signatures of these cells were examined by microarray analysis of 15,814 probes in triplicate experiments. RESULTS: In LNCaP cells, 325 genes were up-regulated by methionine, and 517 genes down-regulated. In MCF-7 cells, 86 genes were up-regulated and 135 genes down-regulated. Ninety-eight genes were regulated in the same direction by methionine in both cells lines, and five other genes were changed in expression in opposite directions. CONCLUSION: Several of the up-regulated genes encode proteins involved in cellular redox regulation, suggesting that methionine may enhance antioxidant mechanisms. Many of the down-regulated genes belong to protein kinase families that may be related to the anti-proliferative effects of methionine on breast and prostate cancer cells.
Authors: Maximo A Benavides; Karen L Hagen; Wenfeng Fang; Pan Du; Simon Lin; Mary P Moyer; Wancai Yang; Kirby I Bland; William E Grizzle; Maarten C Bosland Journal: Anticancer Res Date: 2010-06 Impact factor: 2.480
Authors: Qing Ji; Chisa Aoyama; Yih-Dar Nien; Paul I Liu; Peter K Chen; Lilly Chang; Frank Z Stanczyk; Andrew Stolz Journal: Cancer Res Date: 2004-10-15 Impact factor: 12.701
Authors: Erich Huang; Seiichi Ishida; Jennifer Pittman; Holly Dressman; Andrea Bild; Mark Kloos; Mark D'Amico; Richard G Pestell; Mike West; Joseph R Nevins Journal: Nat Genet Date: 2003-06 Impact factor: 38.330
Authors: Maximo A Benavides; Maarten C Bosland; Cássio P da Silva; Claudia T Gomes Sares; Alana M Cerqueira de Oliveira; Rafael Kemp; Rodolfo B dos Reis; Vilma R Martins; Suely V Sampaio; Kirby I Bland; William E Grizzle; José S dos Santos Journal: Anticancer Drugs Date: 2014-02 Impact factor: 2.248
Authors: Isabelle R Miousse; Julia Tobacyk; Stepan Melnyk; S Jill James; Amrita K Cheema; Marjan Boerma; Martin Hauer-Jensen; Igor Koturbash Journal: Biomol Concepts Date: 2017-05-24