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Literature DB >> 18429073

Protein precipitation using ammonium sulfate.

Abstract

The basic theory of protein precipitation by addition of ammonium sulfate is presented and the most common applications are listed. Numerous tables are provided for calculating the appropriate amount of ammonium sulfate to add to a particular protein solution.

Entities: Chemical

Mesh：

Substances：

Year: 2001 PMID： 18429073 PMCID： PMC4817497 DOI： 10.1002/0471140864.psa03fs13

Source DB: PubMed Journal: Curr Protoc Protein Sci ISSN： 1934-3655

3 in total

1. HIV-1 Rev expressed in recombinant Escherichia coli: purification, polymerization, and conformational properties.

Authors: P T Wingfield; S J Stahl; M A Payton; S Venkatesan; M Misra; A C Steven
Journal: Biochemistry Date: 1991-07-30 Impact factor: 3.162

2. Tables for the preparation of ammonium sulfate solutions.

Authors: W I Wood
Journal: Anal Biochem Date: 1976-05-21 Impact factor: 3.365

3. Effects of sulphate and urea on the stability and reversible unfolding of beta-lactamase from Staphylococcus aureus. Implications for the folding pathway of beta-lactamase.

Authors: C Mitchinson; R H Pain
Journal: J Mol Biol Date: 1985-07-20 Impact factor: 5.469

3 in total

29 in total

1. Deciphering the Function of New Gonococcal Vaccine Antigens Using Phenotypic Microarrays.

Authors: Benjamin I Baarda; Sarah Emerson; Philip J Proteau; Aleksandra E Sikora
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2. General method for rapid purification of native chromatin fragments.

Authors: Vyacheslav I Kuznetsov; Spencer A Haws; Catherine A Fox; John M Denu
Journal: J Biol Chem Date: 2018-05-24 Impact factor: 5.157

3. In vitro and in silico assessment of the developability of a designed monoclonal antibody library.

Authors: Adriana-Michelle Wolf Pérez; Pietro Sormanni; Jonathan Sonne Andersen; Laila Ismail Sakhnini; Ileana Rodriguez-Leon; Jais Rose Bjelke; Annette Juhl Gajhede; Leonardo De Maria; Daniel E Otzen; Michele Vendruscolo; Nikolai Lorenzen
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4. Integration Process for Protein Extraction from Microalgae Using Liquid Biphasic Electric Flotation (LBEF) System.

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5. Binding Affinity Quantifications of the Bacteriophage Mu DNA Modification Protein Mom Using Microscale Thermophoresis (MST).

Authors: Shubha Udupa; Valakunja Nagaraja; Shweta Karambelkar
Journal: Bio Protoc Date: 2022-07-20

Protein precipitation using ammonium sulfate.

1. HIV-1 Rev expressed in recombinant Escherichia coli: purification, polymerization, and conformational properties.

2. Tables for the preparation of ammonium sulfate solutions.

3. Effects of sulphate and urea on the stability and reversible unfolding of beta-lactamase from Staphylococcus aureus. Implications for the folding pathway of beta-lactamase.

1. Deciphering the Function of New Gonococcal Vaccine Antigens Using Phenotypic Microarrays.

2. General method for rapid purification of native chromatin fragments.

3. In vitro and in silico assessment of the developability of a designed monoclonal antibody library.

4. Integration Process for Protein Extraction from Microalgae Using Liquid Biphasic Electric Flotation (LBEF) System.

5. Binding Affinity Quantifications of the Bacteriophage Mu DNA Modification Protein Mom Using Microscale Thermophoresis (MST).

6. Production of hyperimmune serum against genotype VII Newcastle disease virus in rabbits with several applications.

7. Generation of CoilR Probe Peptides for VIPER-labeling of Cellular Proteins.

8. Simple Methods for the Preparation of Colloidal Chitin, Cell Free Supernatant and Estimation of Laminarinase.

9. Nascent RNA scaffolds contribute to chromosome territory architecture and counter chromatin compaction.

10. Measuring myeloperoxidase activity in biological samples.