| Literature DB >> 18428395 |
Bing Yu1, Nicole A Sawyer, Christine Chiu, Peter J Oefner, Peter A Underhill.
Abstract
DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single-base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high-throughput mutation detection and SNP discovery.Mesh：
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Year: 2006 PMID： 18428395 DOI： 10.1002/0471142905.hg0710s48
Source DB: PubMed Journal: Curr Protoc Hum Genet ISSN： 1934-8258