Literature DB >> 18425760

EMS and UV mutagenesis in yeast.

Fred Winston1.   

Abstract

Many fundamental biological processes have been elucidated by the isolation and analysis of mutants that are defective in such processes. Therefore, the methods to generate mutants are of great importance in model organisms. This unit describes two protocols for mutagenesis of yeast-using ethyl methanesulfate (EMS) and ultraviolet (UV) light. Each of these methods has been used successfully for many years. (c) 2008 by John Wiley & Sons, Inc.

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Year:  2008        PMID： 18425760     DOI： 10.1002/0471142727.mb1303bs82

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  12 in total

1.  Activation of the Yeast UBI4 Polyubiquitin Gene by Zap1 Transcription Factor via an Intragenic Promoter Is Critical for Zinc-deficient Growth.

Authors:  Colin W MacDiarmid; Janet Taggart; Jeeyon Jeong; Kittikhun Kerdsomboon; David J Eide
Journal:  J Biol Chem       Date:  2016-07-18       Impact factor: 5.157

2.  Species disparity response to mutagenesis of marine yeasts for the potential production of biodiesel.

Authors:  Thomas Breuck; Boutheina Bessadok; Andrea Santulli; Thomas Brück; Saloua Sadok
Journal:  Biotechnol Biofuels       Date:  2019-05-22       Impact factor: 6.040

3.  Inactivation of a GAL4-like transcription factor improves cell fitness and product yield in glycoengineered Pichia pastoris strains.

Authors:  Bo Jiang; Rebecca Argyros; John Bukowski; Stephanie Nelson; Nathan Sharkey; Sehoon Kim; Victoria Copeland; Robert C Davidson; Ronghua Chen; Jun Zhuang; Natarajan Sethuraman; Terrance A Stadheim
Journal:  Appl Environ Microbiol       Date:  2014-10-24       Impact factor: 4.792

4.  Analysis of Polygenic Mutants Suggests a Role for Mediator in Regulating Transcriptional Activation Distance in Saccharomyces cerevisiae.

Authors:  Caitlin T Reavey; Mark J Hickman; Krista C Dobi; David Botstein; Fred Winston
Journal:  Genetics       Date:  2015-08-17       Impact factor: 4.562

5.  A novel allele of SIR2 reveals a heritable intermediate state of gene silencing.

Authors:  Delaney Farris; Daniel S Saxton; Jasper Rine
Journal:  Genetics       Date:  2021-05-17       Impact factor: 4.562

6.  Genomic saturation mutagenesis and polygenic analysis identify novel yeast genes affecting ethyl acetate production, a non-selectable polygenic trait.

Authors:  Tom Den Abt; Ben Souffriau; Maria R Foulquié-Moreno; Jorge Duitama; Johan M Thevelein
Journal:  Microb Cell       Date:  2016-03-18

7.  Regulation of Yeast-to-Hyphae Transition in Yarrowia lipolytica.

Authors:  Kyle R Pomraning; Erin L Bredeweg; Eduard J Kerkhoven; Kerrie Barry; Sajeet Haridas; Hope Hundley; Kurt LaButti; Anna Lipzen; Mi Yan; Jon K Magnuson; Blake A Simmons; Igor V Grigoriev; Jens Nielsen; Scott E Baker
Journal:  mSphere       Date:  2018-12-05       Impact factor: 4.389

8.  A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin.

Authors:  Gergana Shipkovenska; Alexander Durango; Marian Kalocsay; Steven P Gygi; Danesh Moazed
Journal:  Elife       Date:  2020-06-03       Impact factor: 8.140

9.  Experimental evolution reveals a general role for the methyltransferase Hmt1 in noise buffering.

Authors:  Shu-Ting You; Yu-Ting Jhou; Cheng-Fu Kao; Jun-Yi Leu
Journal:  PLoS Biol       Date:  2019-10-15       Impact factor: 8.029

10.  A phenylalanine to serine substitution within an O-protein mannosyltransferase led to strong resistance to PMT-inhibitors in Pichia pastoris.

Authors:  Rebecca Argyros; Stephanie Nelson; Angela Kull; Ming-Tang Chen; Terrance A Stadheim; Bo Jiang
Journal:  PLoS One       Date:  2013-05-08       Impact factor: 3.240
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