X Li1, G-P He, B Zhang, Y-M Chen, Y-X Su. 1. Department of Nutrition, School of Public Health, Sun Yat-sen University, 74 Zhongshan Rd 2, Guangzhou, 510080, People's Republic of China.
Abstract
UNLABELLED: This study assessed independent associations and interactions of IL-6 promoter alleles (-174G/C and -634C/G), calcium intake and physical activity with bone mass among pre-menarche Chinese girls. The -634 CC carriers, greater calcium intake and physical activity were associated with better bone mass. The gene-bone association was more pronounced among girls with high physical activity or with low calcium intake. INTRODUCTION: The association between interleukin (IL)-6 promoter polymorphisms and bone mass remains in debate. This cross-sectional study examined the association between the IL-6 promoter alleles (-174G/C and -634C/G) and bone mass, and assessed if the association could be modified by calcium intake or physical activity in pre-menarche Chinese girls. METHODS: Two-hundred and twenty-eight healthy pre-menarche girls aged 9-11 years were recruited from primary schools in Guangzhou, China by sending letters to parents. None of them had diseases or medications known to affect bone metabolism. The IL-6 promoter genotypes were determined by PCR-RFLP, and BMD and BMC at the total body, lumbar spine, total hip and femoral neck were measured by DXA. Calcium intake and physical activity were assessed by face-to-face questionnaire interview. RESULTS: One hundred and seventy-six subjects completed the entire study. We did not detect gene polymorphism at the IL-6 -174G/C locus, all were GG homozygotes. The IL-6 -634C/G polymorphism was significantly associated with both BMD and BMC even after adjusting for age and weight. Girls with CC genotype had higher levels of BMC and BMD than G allele carriers (+8.3% for the total body BMC, and +2.9%, +5.8%, and +5.7% for BMDs at the total body, total hip, and femoral neck, respectively; P < 0.05). The favorable effect of physical activity on BMDs at the total hip and femoral neck was much more pronounced in CC carriers than in G allele carriers, and the CC genotype associated higher BMDs at the total hip and femoral neck were observed only in girls with high level physical activity (P for interactions = 0.036 and 0.021, adjusted for age and weight). Calcium had a more benefit to the total body BMC in G allele carriers than in CC carriers, and the G allele-associated lower total body BMC was found only in subjects with low calcium intake. CONCLUSION: The IL-6 -634C/G polymorphism was significantly associated with BMD and the association might be modified by calcium intake or physical activity in pre-menarche Chinese girls.
UNLABELLED: This study assessed independent associations and interactions of IL-6 promoter alleles (-174G/C and -634C/G), calcium intake and physical activity with bone mass among pre-menarche Chinese girls. The -634 CC carriers, greater calcium intake and physical activity were associated with better bone mass. The gene-bone association was more pronounced among girls with high physical activity or with low calcium intake. INTRODUCTION: The association between interleukin (IL)-6 promoter polymorphisms and bone mass remains in debate. This cross-sectional study examined the association between the IL-6 promoter alleles (-174G/C and -634C/G) and bone mass, and assessed if the association could be modified by calcium intake or physical activity in pre-menarche Chinese girls. METHODS: Two-hundred and twenty-eight healthy pre-menarche girls aged 9-11 years were recruited from primary schools in Guangzhou, China by sending letters to parents. None of them had diseases or medications known to affect bone metabolism. The IL-6 promoter genotypes were determined by PCR-RFLP, and BMD and BMC at the total body, lumbar spine, total hip and femoral neck were measured by DXA. Calcium intake and physical activity were assessed by face-to-face questionnaire interview. RESULTS: One hundred and seventy-six subjects completed the entire study. We did not detect gene polymorphism at the IL-6-174G/C locus, all were GG homozygotes. The IL-6-634C/G polymorphism was significantly associated with both BMD and BMC even after adjusting for age and weight. Girls with CC genotype had higher levels of BMC and BMD than G allele carriers (+8.3% for the total body BMC, and +2.9%, +5.8%, and +5.7% for BMDs at the total body, total hip, and femoral neck, respectively; P < 0.05). The favorable effect of physical activity on BMDs at the total hip and femoral neck was much more pronounced in CC carriers than in G allele carriers, and the CC genotype associated higher BMDs at the total hip and femoral neck were observed only in girls with high level physical activity (P for interactions = 0.036 and 0.021, adjusted for age and weight). Calcium had a more benefit to the total body BMC in G allele carriers than in CC carriers, and the G allele-associated lower total body BMC was found only in subjects with low calcium intake. CONCLUSION: The IL-6-634C/G polymorphism was significantly associated with BMD and the association might be modified by calcium intake or physical activity in pre-menarche Chinese girls.
Authors: A Kitamura; G Hasegawa; H Obayashi; K Kamiuchi; M Ishii; M Yano; T Tanaka; M Yamaguchi; H Shigeta; M Ogata; N Nakamura; T Yoshikawa Journal: Diabet Med Date: 2002-12 Impact factor: 4.359
Authors: Jonathan A Mitchell; Alessandra Chesi; Okan Elci; Shana E McCormack; Sani M Roy; Heidi J Kalkwarf; Joan M Lappe; Vicente Gilsanz; Sharon E Oberfield; John A Shepherd; Andrea Kelly; Struan Fa Grant; Babette S Zemel Journal: J Bone Miner Res Date: 2016-08 Impact factor: 6.741
Authors: P F Giampietro; C McCarty; B Mukesh; F McKiernan; D Wilson; A Shuldiner; J Liu; J LeVasseur; L Ivacic; T Kitchner; N Ghebranious Journal: Osteoporos Int Date: 2009-06-09 Impact factor: 4.507