Literature DB >> 18413994

Flavanone 3beta-hydroxylases from rice: key enzymes for favonol and anthocyanin biosynthesis.

Jeong Ho Kim1, Yoon Jung Lee, Bong Gyu Kim, Yoongho Lim, Joong-Hoon Ahn.   

Abstract

Flavanone 3beta-hydroxylases (F3H) are key enzymes in the synthesis of flavonol and anthocyanin. In this study, three F3H cDNAs from Oryza sativa (OsF3H-1 approximately 3) were cloned by RT-PCR and expressed in E. coli as gluthatione S-transferase (GST) fusion proteins. The purified recombinant OsF3Hs used flavanone, naringenin and eriodictyol as substrates. The reaction products with naringen and eriodictyol were determined by nuclear magnetic resonance spectroscopy to be dihydrokaempferol and taxifolin, respectively. OsF3H-1 had the highest enzymatic activity whereas the overall expression of OsF3H-2 was highest in all tissues except seeds. Flavanone 3beta-hydroxylase could be a useful target for flavonoid metabolic engineering in rice.

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Year:  2008        PMID： 18413994

Source DB:  PubMed          Journal:  Mol Cells        ISSN： 1016-8478            Impact factor:   5.034


  8 in total

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Authors:  Xiaoye Shen; Stefan Martens; Mingliang Chen; Daofeng Li; Jiangli Dong; Tao Wang
Journal:  Mol Biol Rep       Date:  2009-11-04       Impact factor: 2.316

2.  Molecular and Biochemical Analysis of Two Rice Flavonoid 3'-Hydroxylase to Evaluate Their Roles in Flavonoid Biosynthesis in Rice Grain.

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3.  Functional Analysis of Two Flavanone-3-Hydroxylase Genes from Camellia sinensis: A Critical Role in Flavonoid Accumulation.

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Authors:  Guoyun Zhang; Daoguo Chen; Tong Zhang; Aiguo Duan; Jianguo Zhang; Caiyun He
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5.  Genome-Wide Association Study of Pericarp Color in Rice Using Different Germplasm and Phenotyping Methods Reveals Different Genetic Architectures.

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Journal:  Sci Rep       Date:  2022-04-15       Impact factor: 4.996

7.  Rerouting plant terpene biosynthesis enables momilactone pathway elucidation.

Authors:  Ricardo De La Peña; Elizabeth S Sattely
Journal:  Nat Chem Biol       Date:  2020-10-26       Impact factor: 15.040

8.  Identification of candidate genes for gelatinization temperature, gel consistency and pericarp color by GWAS in rice based on SLAF-sequencing.

Authors:  Xinghai Yang; Xiuzhong Xia; Yu Zeng; Baoxuan Nong; Zongqiong Zhang; Yanyan Wu; Faqian Xiong; Yuexiong Zhang; Haifu Liang; Guofu Deng; Danting Li
Journal:  PLoS One       Date:  2018-05-10       Impact factor: 3.240
  8 in total

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