Literature DB >> 18408063

In vitro mutagenesis of Bacillus subtilis by using a modified Tn7 transposon with an outward-facing inducible promoter.

Christophe Bordi1, Bronwyn G Butcher, Qiaojuan Shi, Anna-Barbara Hachmann, Joseph E Peters, John D Helmann.   

Abstract

A Tn7 donor plasmid, pTn7SX, was constructed for use with the model gram-positive bacterium Bacillus subtilis. This new mini-Tn7, mTn7SX, contains a spectinomycin resistance cassette and an outward-facing, xylose-inducible promoter, thereby allowing for the regulated expression of genes downstream of the transposon. We demonstrate that mTn7SX inserts are obtained at a high frequency and occur randomly throughout the B. subtilis genome. The utility of this system was demonstrated by the selection of mutants with increased resistance to the antibiotic fosfomycin or duramycin.

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Year:  2008        PMID： 18408063      PMCID： PMC2423030          DOI： 10.1128/AEM.00476-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  50 in total

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4.  Genetic analysis of Bacillus subtilis spo mutations generated by Tn917-mediated insertional mutagenesis.

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Journal:  Genetics       Date:  1987-12       Impact factor: 4.562

5.  Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon.

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6.  Mapping of a genetic locus that affects glycerol 3-phosphate transport in Bacillus subtilis.

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8.  A symmetrical six-base-pair target site sequence determines Tn10 insertion specificity.

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Authors:  E W May; N L Craig
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  9 in total

1.  Tn917 targets the region where DNA replication terminates in Bacillus subtilis, highlighting a difference in chromosome processing in the firmicutes.

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2.  High-frequency transposition for determining antibacterial mode of action.

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Journal:  Nat Chem Biol       Date:  2011-09-04       Impact factor: 15.040

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4.  Mutations in the primary sigma factor σA and termination factor rho that reduce susceptibility to cell wall antibiotics.

Authors:  Yong Heon Lee; John D Helmann
Journal:  J Bacteriol       Date:  2014-08-11       Impact factor: 3.490

5.  Reducing the Level of Undecaprenyl Pyrophosphate Synthase Has Complex Effects on Susceptibility to Cell Wall Antibiotics.

Authors:  Yong Heon Lee; John D Helmann
Journal:  Antimicrob Agents Chemother       Date:  2013-06-24       Impact factor: 5.191

6.  Phenotypic and transcriptomic characterization of Bacillus subtilis mutants with grossly altered membrane composition.

Authors:  Letal I Salzberg; John D Helmann
Journal:  J Bacteriol       Date:  2008-09-26       Impact factor: 3.490

7.  A mutation of the RNA polymerase β' subunit (rpoC) confers cephalosporin resistance in Bacillus subtilis.

Authors:  Yong Heon Lee; Ki Hyun Nam; John D Helmann
Journal:  Antimicrob Agents Chemother       Date:  2012-10-15       Impact factor: 5.191

8.  Transposition into replicating DNA occurs through interaction with the processivity factor.

Authors:  Adam R Parks; Zaoping Li; Qiaojuan Shi; Roisin M Owens; Moonsoo M Jin; Joseph E Peters
Journal:  Cell       Date:  2009-08-21       Impact factor: 41.582

9.  Harnessing the power of transposon mutagenesis for antibacterial target identification and evaluation.

Authors:  Timothy C Meredith; Hao Wang; Patrick Beaulieu; Angelika Gründling; Terry Roemer
Journal:  Mob Genet Elements       Date:  2012-07-01
  9 in total

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