OBJECTIVE: To determine the expression of the genes that code for the isoforms of transforming growth factor-beta (TGF-beta) and TGF-beta receptors (TBR) in mesenchymal stem cells (MSC) from patients with osteoarthritis (OA). METHODS: Total RNA was extracted from primary cultures of MSC and quantitative real-time reverse transcription-polymerase chain reaction was performed to analyze gene expression. RESULTS: MSC from patients with OA showed significantly increased total TGF-beta, TGF-beta1 isoform, TBR-II, and TBR-III mRNA expression compared to controls. CONCLUSION: Our study is the first reporting the gene expression levels of TGF-beta and its isoforms and receptors in patients with OA. These findings might have pathological significance for OA disease.
OBJECTIVE: To determine the expression of the genes that code for the isoforms of transforming growth factor-beta (TGF-beta) and TGF-beta receptors (TBR) in mesenchymal stem cells (MSC) from patients with osteoarthritis (OA). METHODS: Total RNA was extracted from primary cultures of MSC and quantitative real-time reverse transcription-polymerase chain reaction was performed to analyze gene expression. RESULTS: MSC from patients with OA showed significantly increased total TGF-beta, TGF-beta1 isoform, TBR-II, and TBR-III mRNA expression compared to controls. CONCLUSION: Our study is the first reporting the gene expression levels of TGF-beta and its isoforms and receptors in patients with OA. These findings might have pathological significance for OA disease.
Authors: Mildred C Embree; Tina M Kilts; Mitsuaki Ono; Colette A Inkson; Fatima Syed-Picard; Morten A Karsdal; Ake Oldberg; Yanming Bi; Marian F Young Journal: Am J Pathol Date: 2009-12-24 Impact factor: 4.307
Authors: A E Nelson; Y M Golightly; V B Kraus; T Stabler; J B Renner; C G Helmick; J M Jordan Journal: Osteoarthritis Cartilage Date: 2010-02-17 Impact factor: 6.576