Literature DB >> 18389628

[Start codon in the serine proteinase gene from Bacillus intermedius].

A R Kaiumov, A R Sabirova, N P Balaban, A M Mardanova, O N Il'inskaia, S V Kostrov, M R Sharipova.   

Abstract

The translation initiation site in the extracellular serine subtilisin-like proteinase gene from Bacillus intermedius (aprBi) (AN AY754946) secreting at the stationary growth phase was established. The analysis of aprBi open reading frame revealed three putative translation start sites (TTG, GTG, ATG). Using SignalP online freeware program we have determined the functional activity probability of each of them. To identify the translation start point the modified subtilisin-like protease genes carrying nucleotide replacements in supposed start codons were developed using oligonucleotide-directed mutagenesis. We have investigated the expression of these genetic constructions in protease-deficient strain B. subtilis AJ73. According our results it was concluded that the translation in aprBi gene starts from GTG kodon.

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Year:  2008        PMID： 18389628     DOI： 10.1134/s0026893308010172

Source DB:  PubMed          Journal:  Mol Biol (Mosk)        ISSN： 0026-8984


  18 in total

1.  The HIV-1(Lai) RNA dimerization. Thermodynamic parameters associated with the transition from the kissing complex to the extended dimer.

Authors:  V Theilleux-Delalande; F Girard; T Huynh-Dinh; G Lancelot; J Paoletti
Journal:  Eur J Biochem       Date:  2000-05

2.  Structure of the dimer initiation complex of HIV-1 genomic RNA.

Authors:  A Mujeeb; J L Clever; T M Billeci; T L James; T G Parslow
Journal:  Nat Struct Biol       Date:  1998-06

Review 3.  Retroviral RNA dimer linkage.

Authors:  J Greatorex; A Lever
Journal:  J Gen Virol       Date:  1998-12       Impact factor: 3.891

4.  A kissing complex together with a stable dimer is involved in the HIV-1Lai RNA dimerization process in vitro.

Authors:  D Muriaux; P Fossé; J Paoletti
Journal:  Biochemistry       Date:  1996-04-16       Impact factor: 3.162

5.  Conformational constraint as a means for understanding RNA-aminoglycoside specificity.

Authors:  Kenneth F Blount; Fang Zhao; Thomas Hermann; Yitzhak Tor
Journal:  J Am Chem Soc       Date:  2005-07-13       Impact factor: 15.419

6.  A short autocomplementary sequence plays an essential role in avian sarcoma-leukosis virus RNA dimerization.

Authors:  P Fossé; N Motté; A Roumier; C Gabus; D Muriaux; J L Darlix; J Paoletti
Journal:  Biochemistry       Date:  1996-12-24       Impact factor: 3.162

7.  Polymorphism of bulged-out residues in HIV-1 RNA DIS kissing complex and structure comparison with solution studies.

Authors:  Eric Ennifar; Philippe Dumas
Journal:  J Mol Biol       Date:  2005-12-21       Impact factor: 5.469

8.  A loop-loop "kissing" complex is the essential part of the dimer linkage of genomic HIV-1 RNA.

Authors:  J C Paillart; E Skripkin; B Ehresmann; C Ehresmann; R Marquet
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-28       Impact factor: 11.205

9.  A 19-nucleotide sequence upstream of the 5' major splice donor is part of the dimerization domain of human immunodeficiency virus 1 genomic RNA.

Authors:  M Laughrea; L Jetté
Journal:  Biochemistry       Date:  1994-11-15       Impact factor: 3.162

10.  Targeting the dimerization initiation site of HIV-1 RNA with aminoglycosides: from crystal to cell.

Authors:  Eric Ennifar; Jean-Christophe Paillart; Anne Bodlenner; Philippe Walter; Jean-Marc Weibel; Anne-Marie Aubertin; Patrick Pale; Philippe Dumas; Roland Marquet
Journal:  Nucleic Acids Res       Date:  2006-05-05       Impact factor: 16.971
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  1 in total

1.  Regulatory Characteristics of Bacillus pumilus Protease Promoters.

Authors:  Anna A Toymentseva; Thorsten Mascher; Margarita R Sharipova
Journal:  Curr Microbiol       Date:  2017-03-03       Impact factor: 2.188
  1 in total

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