| Literature DB >> 18373226 |
R Rojas-Herrera1, J Narváez-Zapata, M Zamudio-Maya, M E Mena-Martínez.
Abstract
A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.Entities:
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Year: 2008 PMID： 18373226 DOI： 10.1007/s12033-008-9061-8
Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695