| Literature DB >> 18371772 |
Panagiotis Arapitsas1, Charlotta Turner.
Abstract
The aim of this work was to develop a fast method for extraction and analysis of anthocyanins in red cabbage. Pressurized hot water containing 5% of ethanol was used as an extremely efficient extraction solvent. HPLC/DAD with a monolithic column was used to accomplish a fast analysis-24 anthocyanin peaks within 18min. Statistical design was used to optimize the studied extraction parameters: temperature (80-120 degrees C); sample amount (1-3g); extraction time (6-11min); concentration of formic acid in the extraction solvent (0-5vol.%). The best extraction conditions for a majority of the anthocyanin peaks were 2.5g of sample, 99 degrees C (at 50bar), 7min of extraction and a solvent composition of water/ethanol/formic acid (94/5/1, v/v/v).Entities:
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Year: 2007 PMID： 18371772 DOI： 10.1016/j.talanta.2007.08.029
Source DB: PubMed Journal: Talanta ISSN： 0039-9140 Impact factor: 6.057