| Literature DB >> 18371368 |
Kitai Kim1, Kitwa Ng, Peter J Rugg-Gunn, Jae-Hung Shieh, Oktay Kirak, Rudolf Jaenisch, Teruhiko Wakayama, Malcolm A Moore, Roger A Pedersen, George Q Daley.
Abstract
Parthenogenesis and somatic cell nuclear transfer (SCNT) are two methods for deriving embryonic stem (ES) cells that are genetically matched to the oocyte donor or somatic cell donor, respectively. Using genome-wide single nucleotide polymorphism (SNP) analysis, we demonstrate distinct signatures of genetic recombination that distinguish parthenogenetic ES cells from those generated by SCNT. We applied SNP analysis to the human ES cell line SCNT-hES-1, previously claimed to have been derived by SCNT, and present evidence that it represents a human parthenogenetic ES cell line. Genome-wide SNP analysis represents a means to validate the genetic provenance of an ES cell line.Entities:
Mesh：
Year: 2007 PMID： 18371368 DOI： 10.1016/j.stem.2007.07.001
Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633