| Literature DB >> 18370149 |
Kristina Vintersten1, Giuseppe Testa, Ronald Naumann, Konstantinos Anastassiadis, A Francis Stewart.
Abstract
In the mouse, conventional transgenes often produced unpredictable results mainly because they were too small to recapitulate a natural gene context. Bacterial artificial chromosomes (BACs) are large enough to encompass the natural context of most mammalian genes and consequently deliver more reliable recapitulations of their endogenous counterparts. Furthermore, recombineering methods now make it easy to engineer precise changes in a BAC transgene. Consequently, BACs have become the preferred vehicle for mouse transgenesis. Here, we detail methods for BAC transgenesis through pronuclear injection of fertilized oocytes.Entities:
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Year: 2008 PMID： 18370149 DOI： 10.1007/978-1-59745-570-1_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745