| Literature DB >> 25064110 |
Andrei M Vacaru1, Joseph Vitale, Johnathan Nieves, Margaret H Baron.
Abstract
During the development of the hematopoietic system, at least eight distinct lineages are generated in the mouse embryo. Transgenic mice expressing fluorescent proteins at various points in the hematopoietic hierarchy, from hematopoietic stem cell to multipotent progenitors to each of the final differentiated cell types, have provided valuable tools for tagging, tracking, and isolating these cells. In this chapter, we discuss general considerations in designing a transgene and survey available fluorescent probes and methods for confirming and analyzing transgene expression in the hematopoietic systems of the embryo, fetus, and postnatal/adult animal.Entities:
Mesh：
Substances：
Year: 2014 PMID： 25064110 PMCID： PMC4418647 DOI： 10.1007/978-1-4939-1215-5_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745