| Literature DB >> 18359519 |
Chen Hongyun1, Zhao Wenjun, Gu Qinsheng, Chen Qing, Lin Shiming, Zhu Shuifang.
Abstract
A real time reverse-transcription polymerase chain reaction (RT-PCR) was developed for efficient detection of Cucumber green mottle mosaic virus (CGMMV). The method was designed to use a duo-primer system with a TaqMan probe targeting the conserved sequence in 3' noncoding region (NCR) of CGMMV to detect isolates of this virus collected in China. The sensitivity of the real time RT-PCR assay was 0.13 pg of total RNA or 50 molecules of RNA transcripts. This level of sensitivity indicated that the one step real time RT-PCR developed in the present study could be used for routine testing assays. The real time RT-PCR method could assist in the implementation of quarantine measures for prevention and control of the disease caused by CGMMV.Entities:
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Year: 2008 PMID： 18359519 DOI： 10.1016/j.jviromet.2008.02.006
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014