BACKGROUND: HTLV-1/2 diagnosis in high-risk populations from São Paulo, Brazil has been problematic due a high proportion of seroindeterminate results. OBJECTIVES: To confirm and extend previous findings regarding HTLV-1/2 diagnosis in this geographic area. STUDY DESIGN: Sera from 2312 patients were tested for HTLV-1/2 antibodies using enzyme immunoassay (EIA) and Western blot (WB) analysis. Patients were from AIDS Reference Centers (Group I; 1393 patients) and HTLV out-patient clinics (Group II; 919 patients). Results were analyzed according to patients' age, gender, and clinic type. RESULTS: HTLV-1 and HTLV-2 were detected in both groups. Among seropositive females, HTLV-2 was slightly more common in Group I (54.5%), while HTLV-1 prevailed in Group II (73.9%). Males from Group II had a higher percentage of HTLV-seroindeterminate results. No correlation between HTLV serological results and age was detected. Temporal analyses disclosed a high number of HTLV-seroindeterminate samples, and a large spectrum of indeterminate WB profiles. GD21 and/or rgp46-II bands were detected in 34.6% of sera from Group I, and a p24 or p19 band was detected in 35.3% of sera from Group II. CONCLUSIONS: High rates of HTLV-indeterminate serological patterns during temporal analyses were confirmed in high-risk populations from São Paulo, Brazil.
BACKGROUND:HTLV-1/2 diagnosis in high-risk populations from São Paulo, Brazil has been problematic due a high proportion of seroindeterminate results. OBJECTIVES: To confirm and extend previous findings regarding HTLV-1/2 diagnosis in this geographic area. STUDY DESIGN: Sera from 2312 patients were tested for HTLV-1/2 antibodies using enzyme immunoassay (EIA) and Western blot (WB) analysis. Patients were from AIDS Reference Centers (Group I; 1393 patients) and HTLV out-patient clinics (Group II; 919 patients). Results were analyzed according to patients' age, gender, and clinic type. RESULTS:HTLV-1 and HTLV-2 were detected in both groups. Among seropositive females, HTLV-2 was slightly more common in Group I (54.5%), while HTLV-1 prevailed in Group II (73.9%). Males from Group II had a higher percentage of HTLV-seroindeterminate results. No correlation between HTLV serological results and age was detected. Temporal analyses disclosed a high number of HTLV-seroindeterminate samples, and a large spectrum of indeterminate WB profiles. GD21 and/or rgp46-II bands were detected in 34.6% of sera from Group I, and a p24 or p19 band was detected in 35.3% of sera from Group II. CONCLUSIONS: High rates of HTLV-indeterminate serological patterns during temporal analyses were confirmed in high-risk populations from São Paulo, Brazil.
Authors: Aldemir Branco Oliveira-Filho; Paula Cristina Rodrigues Frade; Ricardo Roberto de Souza Fonseca; Leila Sawada; Luísa Caricio Martins; Luiz Fernando Almeida Machado; Antonio Carlos Rosário Vallinoto; Ricardo Ishak; José Alexandre Rodrigues de Lemos; Benedikt Fischer; Emil Kupek Journal: Front Microbiol Date: 2022-06-03 Impact factor: 6.064
Authors: Adele Caterino-de-Araujo; Mariana Cavalheiro Magri; Neuza Satomi Sato; Helena Kaminami Morimoto; Luis Fernando de Macedo Brigido; Arilson Akira Morimoto Journal: AIDS Res Hum Retroviruses Date: 2013-08-19 Impact factor: 2.205
Authors: Karoline R Campos; Fred L N Santos; Bernardo Galvão-Castro; Adele Caterino-de-Araujo; Vanessa da Silva Brito; Noilson L S Gonçalves; Thessika H A Araujo Journal: J Clin Microbiol Date: 2019-12-23 Impact factor: 5.948