| Literature DB >> 18346456 |
Myoung Sook Kim1, Cinthia Lebron, Jatin K Nagpal, Young Kwang Chae, Xiaofei Chang, Yiping Huang, Tony Chuang, Keishi Yamashita, Barry Trink, Edward A Ratovitski, Joseph A Califano, David Sidransky.
Abstract
The pathogenesis of breast cancer involves multiple genetic and epigenetic events. In this study, we report an epigenetic alteration of DFNA5 in human breast cancer. DFNA5 gene was silenced in breast cancer cell lines that were methylated in the DFNA5 promoter, and restored by treatment with the demethylating agent, 5-aza-dC, and gene knock-down of DFNA5 increased cellular invasiveness in vitro. The mRNA expression of DFNA5 in breast cancer tissues was down-regulated as compared to normal tissues. Moreover, the DFNA5 promoter was found to be methylated in primary tumor tissues with high frequency (53%, 18/34). Quantitative methylation-specific PCR of DFNA5 clearly discriminated primary breast cancer tissues from normal breast tissues (15.3%, 2/13). Moreover, methylation status of DFNA5 was correlated with lymph node metastasis in breast cancer patients. Our data implicate DFNA5 promoter methylation as a novel molecular biomarker in human breast cancer.Entities:
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Year: 2008 PMID： 18346456 PMCID： PMC3094717 DOI： 10.1016/j.bbrc.2008.03.026
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575