INTRODUCTION: Although there is considerable variability in methodology among umbilical cord blood banks, their common goal is to achieve optimal product quality for transplantation. Cryopreservation is a critical issue for a long-term maintenance of cord blood viability and colony-forming capacities. MATERIALS AND METHODS: We designed a prospective study to compare controlled (CRF) vs. non-controlled freezing (URF) of volume-reduced cord blood units. In addition, the influence of hydroxy ethyl starch (HES) on cryopreservation was also assayed. To assess the efficiency of protocols used, cell recoveries were measured and the presence of hematopoietic colony-forming units was quantified. RESULTS: In the study phase, we observed similar CB haematopoietc recoveries for CRF and URF strategies, except for TNC recovery that was better for HES volume reduced CB units in the URF group. When we analysed the data of routine processed CB units in samples from satellite cryovials, we found better BFU-E, CFU-GM, CFU-GEMM and CFU recoveries for those units processed with HES than without HES, in an URF manner. CONCLUSIONS: URF of CB units is a cryopreservation procedure that allows similar hematopoietic progenitor recoveries than CRF with programmed devices. However, our study suggests that those banks that cryopreserve CB units in a URF manner should use HES for volume reduction. On the other hand, for CRF cryopreservation methodology volume reduction with and without HES are equally useful.
INTRODUCTION: Although there is considerable variability in methodology among umbilical cord blood banks, their common goal is to achieve optimal product quality for transplantation. Cryopreservation is a critical issue for a long-term maintenance of cord blood viability and colony-forming capacities. MATERIALS AND METHODS: We designed a prospective study to compare controlled (CRF) vs. non-controlled freezing (URF) of volume-reduced cord blood units. In addition, the influence of hydroxy ethyl starch (HES) on cryopreservation was also assayed. To assess the efficiency of protocols used, cell recoveries were measured and the presence of hematopoietic colony-forming units was quantified. RESULTS: In the study phase, we observed similar CB haematopoietc recoveries for CRF and URF strategies, except for TNC recovery that was better for HES volume reduced CB units in the URF group. When we analysed the data of routine processed CB units in samples from satellite cryovials, we found better BFU-E, CFU-GM, CFU-GEMM and CFU recoveries for those units processed with HES than without HES, in an URF manner. CONCLUSIONS: URF of CB units is a cryopreservation procedure that allows similar hematopoietic progenitor recoveries than CRF with programmed devices. However, our study suggests that those banks that cryopreserve CB units in a URF manner should use HES for volume reduction. On the other hand, for CRF cryopreservation methodology volume reduction with and without HES are equally useful.
Authors: Mukta S Sane; Neha Misra; Omid Mohammad Mousa; Steve Czop; Huiyuan Tang; Larry T Khoo; Christopher D Jones; Soumyajit Banerjee Mustafi Journal: Regen Med Date: 2018-10-22 Impact factor: 3.806
Authors: Jun Hayakawa; Elizabeth G Joyal; Jean F Gildner; Kareem N Washington; Oswald A Phang; Naoya Uchida; Matthew M Hsieh; John F Tisdale Journal: Transfusion Date: 2010-10-04 Impact factor: 3.157
Authors: Rowayda Peters; Monika J Wolf; Maries van den Broek; Mario Nuvolone; Stefanie Dannenmann; Bruno Stieger; Reto Rapold; Daniel Konrad; Arnold Rubin; Joseph R Bertino; Adriano Aguzzi; Mathias Heikenwalder; Alexander K Knuth Journal: PLoS One Date: 2010-12-30 Impact factor: 3.240
Authors: Olga Margareth Wanderley de Oliveira Félix; Gisela Tunes; Valéria Cortez Ginani; Paulo César Simões; Daniele Porto Barros; Elizabete Delbuono; Maria Teresa de Seixas Alves; Antonio Sérgio Petrilli; Maria Lúcia de Martino Lee; Roseane Vasconcelos Gouveia; Victor Gottardello Zecchin; Adriana Seber Journal: Hematol Transfus Cell Ther Date: 2018-03-24