Mukta S Sane1, Neha Misra1, Omid Mohammad Mousa1,2, Steve Czop3, Huiyuan Tang1, Larry T Khoo4, Christopher D Jones1,3, Soumyajit Banerjee Mustafi1. 1. Department of Research & Development, Burst Biologics, Boise, ID 83705, USA. 2. Biomolecular Research Center, Boise State University, Boise, ID, 83725, USA. 3. Department of Regulatory & Medical Affairs, Burst Biologics, Boise, ID 83705, USA. 4. The Spine Clinic of Los Angeles, Good Samaritan Hospital, University of Southern California, Los Angeles, CA 90017, USA.
Abstract
AIM: Umbilical cord blood (UCB) finds frequent applications in regenerative medicine. We evaluated the role of cytokines present in a uniquely processed, UCB-derived cellular allograft product (UCBp). MATERIALS & METHODS: Luminex multiplex assay and standard cell biology methods were employed. RESULTS: Study with allografts from 33 donors identified 44 quantifiable cytokines in the UCBp derived conditioned media (CM). The UCBp-CM elevated proliferation and migration rates of mesenchymal stem cells (MSCs) and bone marrow stromal cells. Moreover, UCBp-CM induced secretion of VEGF-A and osteoprotegerin, which promoted angiogenesis of endothelial cells and positively influenced the osteogenic differentiation of MSCs, respectively. CONCLUSION: Cytokines in UCBp stimulate cellular processes important for bone regeneration, making UCBp an excellent candidate for potential applications in orthopedic procedures like bone non-union and spinal fusion.
AIM: Umbilical cord blood (UCB) finds frequent applications in regenerative medicine. We evaluated the role of cytokines present in a uniquely processed, UCB-derived cellular allograft product (UCBp). MATERIALS & METHODS: Luminex multiplex assay and standard cell biology methods were employed. RESULTS: Study with allografts from 33 donors identified 44 quantifiable cytokines in the UCBp derived conditioned media (CM). The UCBp-CM elevated proliferation and migration rates of mesenchymal stem cells (MSCs) and bone marrow stromal cells. Moreover, UCBp-CM induced secretion of VEGF-A and osteoprotegerin, which promoted angiogenesis of endothelial cells and positively influenced the osteogenic differentiation of MSCs, respectively. CONCLUSION: Cytokines in UCBp stimulate cellular processes important for bone regeneration, making UCBp an excellent candidate for potential applications in orthopedic procedures like bone non-union and spinal fusion.
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