Literature DB >> 18337392

Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species.

H Moyaert1, F Pasmans, R Ducatelle, F Haesebrouck, M Baele.   

Abstract

The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.

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Year:  2008        PMID： 18337392      PMCID： PMC2395105          DOI： 10.1128/JCM.00139-08

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

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4.  Identification of enterohepatic Helicobacter species by restriction fragment-length polymorphism analysis of the 16S rRNA gene.

Authors:  Z Shen; Y Feng; J G Fox
Journal:  Helicobacter       Date:  2000-09       Impact factor: 5.753

5.  Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov.

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Authors:  Stephen L W On; Penelope J Jordan
Journal:  J Clin Microbiol       Date:  2003-01       Impact factor: 5.948

9.  Strategy for the detection of Helicobacter species by amplification of 16S rRNA genes and identification of H. felis in a human gastric biopsy.

Authors:  Y Germani; C Dauga; P Duval; M Huerre; M Levy; G Pialoux; P Sansonetti; P A Grimont
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  8 in total

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6.  Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern.

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7.  Helicobacter species in cancers of the gallbladder and extrahepatic biliary tract.

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8.  SpeciesPrimer: a bioinformatics pipeline dedicated to the design of qPCR primers for the quantification of bacterial species.

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  8 in total

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