| Literature DB >> 18337085 |
Nobuyoshi Kittaka1, Ichiro Takemasa, Yutaka Takeda, Shigeru Marubashi, Hiroaki Nagano, Koji Umeshita, Keizo Dono, Kenichi Matsubara, Nariaki Matsuura, Morito Monden.
Abstract
DNA microarray analysis of human cancer has resulted in considerable accumulation of global gene profiles. However, extraction and understanding the underlying biology of cancer progression remains a significant challenge. This study applied a novel integrative computational and analytical approach to this challenge in human hepatocellular carcinoma (HCC) with the aim of identifying potential molecular markers or novel therapeutic targets. We analysed 100 HCC tissue samples by human 30K DNA microarray. The gene expression data were uploaded into the network analysis tool, and the biological networks were displayed graphically. We identified several activated 'hotspot' regions harbouring a concentration of upregulated genes. Several 'hotspot' regions revealed integrin and Akt/NF-kappaB signalling. We identified key members linked to these signalling pathways including osteopontin (SPP1), glypican-3 (GPC3), annexin 2 (ANXA2), S100A10 and vimentin (VIM). Our integrative approach should significantly enhance the power of microarray data in identifying novel potential targets in human cancer.Entities:
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Year: 2008 PMID： 18337085 DOI： 10.1016/j.ejca.2008.02.019
Source DB: PubMed Journal: Eur J Cancer ISSN： 0959-8049 Impact factor: 9.162