BACKGROUND: Airway epithelial cells have shown to be active participants in the defense against pathogens by producing signaling and other regulatory molecules in response to the encounter. METHODS: In previous manuscripts, we have studied the effect of house dust mite (HDM) extract on both an epithelial cell-line (H292) and primary nasal epithelial cell. When we compare these responses we conclude that the H292 cells more closely resemble nasal epithelium of healthy controls (share 107 probe-sets) than of allergic individuals (share 17 probe-sets). RESULTS: Interestingly, probably because of an absent intraindividual variation between samples, more probe-sets (8280) change expression significantly in H292 than in either healthy (555) or allergic (401) epithelium. CONCLUSIONS: A direct comparison of all the responses in these epithelial cells reveals a core-response to HDM of just 29 genes. These genes (CCL20, IL-8, CXCL2, CXCL1, IL-1B, AREG, TNFAIP3, HBEGF, PTGS2, BMP2, LDLR, PLAUR, PLAU, NFKB2, NFKB1, JUN, ATF3, EGR1, NPC1, TICAM1, EPHA2, CTGF, DUSP1, SPRY1, TLR-3, complement factor C3, IVNS1ABP, SerpinB3, and PSAT1) have described links with allergy or inflammation and may even describe the well-established relationship between viral infections and allergic exacerbations or allergy development.
BACKGROUND: Airway epithelial cells have shown to be active participants in the defense against pathogens by producing signaling and other regulatory molecules in response to the encounter. METHODS: In previous manuscripts, we have studied the effect of house dust mite (HDM) extract on both an epithelial cell-line (H292) and primary nasal epithelial cell. When we compare these responses we conclude that the H292 cells more closely resemble nasal epithelium of healthy controls (share 107 probe-sets) than of allergic individuals (share 17 probe-sets). RESULTS: Interestingly, probably because of an absent intraindividual variation between samples, more probe-sets (8280) change expression significantly in H292 than in either healthy (555) or allergic (401) epithelium. CONCLUSIONS: A direct comparison of all the responses in these epithelial cells reveals a core-response to HDM of just 29 genes. These genes (CCL20, IL-8, CXCL2, CXCL1, IL-1B, AREG, TNFAIP3, HBEGF, PTGS2, BMP2, LDLR, PLAUR, PLAU, NFKB2, NFKB1, JUN, ATF3, EGR1, NPC1, TICAM1, EPHA2, CTGF, DUSP1, SPRY1, TLR-3, complement factor C3, IVNS1ABP, SerpinB3, and PSAT1) have described links with allergy or inflammation and may even describe the well-established relationship between viral infections and allergic exacerbations or allergy development.
Authors: K I L Röschmann; A-M van Kuijen; S Luiten; M J Jonker; T M Breit; W J Fokkens; A Petersen; C M van Drunen Journal: Clin Exp Immunol Date: 2012-03 Impact factor: 4.330
Authors: Stewart T G Burgess; David Frew; Francesca Nunn; Craig A Watkins; Tom N McNeilly; Alasdair J Nisbet; John F Huntley Journal: BMC Genomics Date: 2010-11-10 Impact factor: 3.969
Authors: Stewart T G Burgess; Tom N McNeilly; Craig A Watkins; Alasdair J Nisbet; John F Huntley Journal: PLoS One Date: 2011-09-07 Impact factor: 3.240
Authors: M Rochman; A V Kartashov; J M Caldwell; M H Collins; E M Stucke; K Kc; J D Sherrill; J Herren; A Barski; M E Rothenberg Journal: Mucosal Immunol Date: 2014-11-12 Impact factor: 7.313
Authors: Korneliusz Golebski; Silvia Luiten; Danielle van Egmond; Esther de Groot; Kristina Irene Lisolette Röschmann; Wytske Johanna Fokkens; Cornelis Maria van Drunen Journal: PLoS One Date: 2014-02-03 Impact factor: 3.240