BACKGROUND: Dengue and Japanese encephalitis viruses co-circulate in Thailand. IgM-capture enzyme-linked immunosorbent assay (ELISA) has been widely used for confirmation of dengue and Japanese encephalitis (JE). OBJECTIVES: To examine the cross-reactivity in IgM responses to dengue and JE viruses in serum and CSF samples from dengue and JE patients. STUDY DESIGN: Two hundred and fifty-eight serum samples from 177 confirmed dengue patients, and 99 serum samples and 37 cerebrospinal fluid (CSF) samples from confirmed JE patients were analyzed. RESULTS: Nine percent of serum samples from dengue patients were positive for anti-JE IgM. Thirteen percent of serum samples and 11% of CSF samples from JE patients were positive for anti-dengue IgM. Levels of cross-reactive IgM were lower than those of specific IgM in all the dengue and JE patients. CONCLUSIONS: Only specific IgM is detected in about 90% of dengue and JE patients, but cross-reactive IgM is also detected in the remainder. The presence of cross-reactive IgM responses should to be considered in the serodiagnosis of dengue and JE, especially in areas where dengue and JE viruses co-circulate.
BACKGROUND: Dengue and Japanese encephalitis viruses co-circulate in Thailand. IgM-capture enzyme-linked immunosorbent assay (ELISA) has been widely used for confirmation of dengue and Japanese encephalitis (JE). OBJECTIVES: To examine the cross-reactivity in IgM responses to dengue and JE viruses in serum and CSF samples from dengue and JEpatients. STUDY DESIGN: Two hundred and fifty-eight serum samples from 177 confirmed dengue patients, and 99 serum samples and 37 cerebrospinal fluid (CSF) samples from confirmed JEpatients were analyzed. RESULTS: Nine percent of serum samples from dengue patients were positive for anti-JE IgM. Thirteen percent of serum samples and 11% of CSF samples from JEpatients were positive for anti-dengue IgM. Levels of cross-reactive IgM were lower than those of specific IgM in all the dengue and JEpatients. CONCLUSIONS: Only specific IgM is detected in about 90% of dengue and JEpatients, but cross-reactive IgM is also detected in the remainder. The presence of cross-reactive IgM responses should to be considered in the serodiagnosis of dengue and JE, especially in areas where dengue and JE viruses co-circulate.
Authors: Jaimie S Robinson; David Featherstone; Ravi Vasanthapuram; Brad J Biggerstaff; Anita Desai; Nalini Ramamurty; Anwarul Haque Chowdhury; Hardeep S Sandhu; Kathleen F Cavallaro; Barbara W Johnson Journal: Am J Trop Med Hyg Date: 2010-11 Impact factor: 2.345
Authors: James A Potts; Stephen J Thomas; Anon Srikiatkhachorn; Pra-on Supradish; Wenjun Li; Ananda Nisalak; Suchitra Nimmannitya; Timothy P Endy; Daniel H Libraty; Robert V Gibbons; Sharone Green; Alan L Rothman; Siripen Kalayanarooj Journal: Am J Trop Med Hyg Date: 2010-10 Impact factor: 2.345
Authors: Jean David Pommier; Chris Gorman; Yoann Crabol; Kevin Bleakley; Heng Sothy; Ky Santy; Huong Thi Thu Tran; Lam Van Nguyen; Em Bunnakea; Chaw Su Hlaing; Aye Mya Min Aye; Julien Cappelle; Magali Herrant; Patrice Piola; Bruno Rosset; Veronique Chevalier; Arnaud Tarantola; Mey Channa; Jerome Honnorat; Anne Laure Pinto; Sayaphet Rattanavong; Manivanh Vongsouvath; Mayfong Mayxay; Sommanikhone Phangmanixay; Khounthavy Phongsavath; Ommar Swe Tin; Latt Latt Kyaw; Htay Htay Tin; Kyaw Linn; Thi Mai Hung Tran; Philippe Pérot; Nguyen Thi Thu Thuy; Nguyen Hien; Phuc Huu Phan; Philippe Buchy; Philippe Dussart; Denis Laurent; Marc Eloit; Audrey Dubot-Pérès; Olivier Lortholary; Xavier de Lamballerie; Paul N Newton; Marc Lecuit Journal: Lancet Glob Health Date: 2022-07 Impact factor: 38.927
Authors: Jaya S Kshatri; Matrujyoti Pattnaik; Goldi Badaik; Hari R Choudhary; Asit Mansingh; Sanghamitra Pati; Debdutta Bhattacharya Journal: J Family Med Prim Care Date: 2022-05-14
Authors: Patricia L Schirmer; Cynthia A Lucero-Obusan; Stephen R Benoit; Luis M Santiago; Danielle Stanek; Achintya Dey; Mirsonia Martinez; Gina Oda; Mark Holodniy Journal: PLoS Negl Trop Dis Date: 2013-03-14