Johann D D Pitout1, Yi Wei, Deirdre L Church, Daniel B Gregson. 1. Division of Microbiology, Calgary Laboratory Services, University of Calgary, #9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8. johann.pitout@cls.ab.ca
Abstract
OBJECTIVES: A study was designed to determine the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants among clinical isolates of Enterobacteriaceae from the Calgary Health Region (CHR). METHODS: During January and February 2004 and January and February 2007, 564 non-repeat isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis and Morganella morganii resistant to ciprofloxacin and/or tobramycin were screened for PMQR determinants using a multiplex PCR for qnrA, qnrS and qnrB and aac(6')-Ib genes; aac(6')-Ib-cr was further identified by digestion with BstF5I. RESULTS: In 2004, 6/139 (4%) of the resistant E. coli were positive for aac(6')-Ib-cr. In 2007, 53/398 (13%) were positive for aac(6')-Ib-cr, 3/398 (0.8%) were positive for qnrS, and one isolate was positive for both. All the isolates were present in urines and the majority [40/63 (63%)] were submitted from community collection sites; 8 (13%) isolates co-produced AmpC beta-lactamases and 34 (54%) co-produced CTX-M-15. aac(6')-Ib-cr was present in one Klebsiella pneumoniae and one P. mirabilis, whereas one isolate of K. pneumoniae was positive for both aac(6')-Ib-cr and qnrB. CONCLUSIONS: Our results showed that isolates with aac(6')-Ib-cr, often associated with CTX-M-15, are emerging among fluoroquinolone-resistant E. coli in the CHR. Our study suggests that surveillance for PMQR determinants should be undertaken on a regular basis.
OBJECTIVES: A study was designed to determine the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants among clinical isolates of Enterobacteriaceae from the Calgary Health Region (CHR). METHODS: During January and February 2004 and January and February 2007, 564 non-repeat isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis and Morganella morganii resistant to ciprofloxacin and/or tobramycin were screened for PMQR determinants using a multiplex PCR for qnrA, qnrS and qnrB and aac(6')-Ib genes; aac(6')-Ib-cr was further identified by digestion with BstF5I. RESULTS: In 2004, 6/139 (4%) of the resistant E. coli were positive for aac(6')-Ib-cr. In 2007, 53/398 (13%) were positive for aac(6')-Ib-cr, 3/398 (0.8%) were positive for qnrS, and one isolate was positive for both. All the isolates were present in urines and the majority [40/63 (63%)] were submitted from community collection sites; 8 (13%) isolates co-produced AmpC beta-lactamases and 34 (54%) co-produced CTX-M-15. aac(6')-Ib-cr was present in one Klebsiella pneumoniae and one P. mirabilis, whereas one isolate of K. pneumoniae was positive for both aac(6')-Ib-cr and qnrB. CONCLUSIONS: Our results showed that isolates with aac(6')-Ib-cr, often associated with CTX-M-15, are emerging among fluoroquinolone-resistant E. coli in the CHR. Our study suggests that surveillance for PMQR determinants should be undertaken on a regular basis.
Authors: Patricia J Baudry-Simner; Amanpreet Singh; James A Karlowsky; Daryl J Hoban; George G Zhanel Journal: Can J Infect Dis Med Microbiol Date: 2012 Impact factor: 2.471
Authors: Gabriela Warburg; Maya Korem; Ari Robicsek; Dalia Engelstein; Allon E Moses; Colin Block; Jacob Strahilevitz Journal: Antimicrob Agents Chemother Date: 2008-12-22 Impact factor: 5.191
Authors: Le Thi Minh Vien; Manal Abuoun; Victoria Morrison; Nicholas Thomson; James I Campbell; Martin J Woodward; Nguyen Van Vinh Chau; Jeremy Farrar; Constance Schultsz; Stephen Baker Journal: Antimicrob Agents Chemother Date: 2011-01-31 Impact factor: 5.191
Authors: Maria Rosa Anna Plano; Anna Maria Di Noto; Alberto Firenze; Sonia Sciortino; Caterina Mammina Journal: Interdiscip Perspect Infect Dis Date: 2009-09-10