OBJECTIVE: To determine the optimum time interval between semen processing and incubation before intracytoplasmic sperm injection (ICSI) and correlate it with the acrosomal reaction rate. DESIGN: Controlled randomized study. SETTING: The Egyptian IVF-ET Center. PATIENT(S): Couples with male factor infertility undergoing ICSI using ejaculated semen. INTERVENTION(S): The patients were prospectively randomized according to differences in sperm preincubation time before ICSI into 1-hour, 3-hour, and 5-hour groups. The status of the acrosome was studied using electron microscopy. MAIN OUTCOME MEASURE(S): The primary outcome measures were fertilization rate and acrosome reaction rate. Secondary outcome measures were the implantation and pregnancy rates. RESULT(S): The rate of acrosomally reacted spermatozoa was the highest (68.2%) after 5 hours of incubation and lowest (25.6%) after 1 hour of incubation. The difference was statistically significant. The fertilization rate was the highest (74%) using spermatozoa incubated for 3 hours as compared with 1 hour (70%) and 5 hours (67%), but the difference was not statistically significant. CONCLUSION(S): Acrosome reaction is time dependent; the optimum incubation time of spermatozoa before ICSI was 3 hours, which resulted in the highest fertilization rate.
RCT Entities:
OBJECTIVE: To determine the optimum time interval between semen processing and incubation before intracytoplasmic sperm injection (ICSI) and correlate it with the acrosomal reaction rate. DESIGN: Controlled randomized study. SETTING: The Egyptian IVF-ET Center. PATIENT(S): Couples with male factor infertility undergoing ICSI using ejaculated semen. INTERVENTION(S): The patients were prospectively randomized according to differences in sperm preincubation time before ICSI into 1-hour, 3-hour, and 5-hour groups. The status of the acrosome was studied using electron microscopy. MAIN OUTCOME MEASURE(S): The primary outcome measures were fertilization rate and acrosome reaction rate. Secondary outcome measures were the implantation and pregnancy rates. RESULT(S): The rate of acrosomally reacted spermatozoa was the highest (68.2%) after 5 hours of incubation and lowest (25.6%) after 1 hour of incubation. The difference was statistically significant. The fertilization rate was the highest (74%) using spermatozoa incubated for 3 hours as compared with 1 hour (70%) and 5 hours (67%), but the difference was not statistically significant. CONCLUSION(S): Acrosome reaction is time dependent; the optimum incubation time of spermatozoa before ICSI was 3 hours, which resulted in the highest fertilization rate.
Authors: Annalisa De Angelis; Maria Antonietta Ferrara; Gianfranco Coppola; Loredana Di Matteo; Laura Siani; Brian Dale; Giuseppe Coppola; Anna Chiara De Luca Journal: Sci Rep Date: 2019-03-18 Impact factor: 4.379
Authors: Paula Sáez-Espinosa; Natalia Huerta-Retamal; Laura Robles-Gómez; Manuel Avilés; Jon Aizpurua; Irene Velasco; Alejandro Romero; María José Gómez-Torres Journal: Asian J Androl Date: 2020 Sep-Oct Impact factor: 3.285