Literature DB >> 18294837

Amperometric immunosensor for diagnosis of BLV infection.

Bogumila Kurtinaitiene1, Dovile Ambrozaite, Valdas Laurinavicius, Almira Ramanaviciene, Arunas Ramanavicius.   

Abstract

A new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The detection of antibody-antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N',N'-tetramethylbenzidine (TMB) were selected as suitable mediators for this immunosensor. Optimal conditions for amperometric detection were found. Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.

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Year:  2008        PMID： 18294837     DOI： 10.1016/j.bios.2008.01.014

Source DB:  PubMed          Journal:  Biosens Bioelectron        ISSN： 0956-5663            Impact factor:   10.618


  6 in total

Review 1.  Electrochemical sensors.

Authors:  Benjamin J Privett; Jae Ho Shin; Mark H Schoenfisch
Journal:  Anal Chem       Date:  2010-06-15       Impact factor: 6.986

2.  Electrochemical immunosensor for the breast cancer marker CA 15-3 based on the catalytic activity of a CuS/reduced graphene oxide nanocomposite towards the electrooxidation of catechol.

Authors:  Jafar Amani; Alireza Khoshroo; Mehdi Rahimi-Nasrabadi
Journal:  Mikrochim Acta       Date:  2017-12-26       Impact factor: 5.833

3.  Flow-through microfluidic immunosensors with refractive index-matched silica monoliths as volumetric optical detection elements.

Authors:  M S Wiederoder; E L Kendall; J-H Han; R G Ulrich; D L DeVoe
Journal:  Sens Actuators B Chem       Date:  2017-07-21       Impact factor: 7.460

4.  Optical detection enhancement in porous volumetric microfluidic capture elements using refractive index matching fluids.

Authors:  M S Wiederoder; L Peterken; A X Lu; O D Rahmanian; S R Raghavan; D L DeVoe
Journal:  Analyst       Date:  2015-08-21       Impact factor: 4.616

5.  Interaction mechanism between TiO2 nanostructures and bovine leukemia virus proteins in photoluminescence-based immunosensors.

Authors:  Alla Tereshchenko; Valentyn Smyntyna; Arunas Ramanavicius
Journal:  RSC Adv       Date:  2018-11-09       Impact factor: 4.036

Review 6.  Advances in Electrochemical and Acoustic Aptamer-Based Biosensors and Immunosensors in Diagnostics of Leukemia.

Authors:  Tibor Hianik
Journal:  Biosensors (Basel)       Date:  2021-05-31
  6 in total

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