PURPOSE: To apply Polymerase Chain Reaction (PCR)-based DNA sequencing targeting Internal Transcribed Spacer (ITS) region for identification of non-sporulating molds (NSM) to species level which formed 12% of ocular isolates of fungi in a tertiary eye hospital in South India. MATERIALS AND METHODS: Fifty ocular filamentous fungal NSM isolates recovered from 45 patients were included in the study. PCR-based DNA sequencing technique targeting ITS region was applied to identify NSM. RESULTS: PCR-based DNA sequencing revealed 23 established pathogens involving 8 genera (Aspergillus, Fusarium, Bipolaris, Pythium, Cochilobolus, Exserohilum, Pseudoallescheria, and Scedosporiumspecies) and 27 emerging pathogens involving 7 genera (Botryosphaeria Lasiodiplodia species, Thielavia tortuosa, Glomerulla singulata, Macrophomina phaseolina, Rhizoctonia bataticola, and Podosporaspecies) reported for the first time in literature related to ocular infections. Fifteen (30%) patients with fungal keratitis caused by NSM failed to respond to standard antifungal therapy. CONCLUSION: PCR-based DNA sequencing technique is a rapid, reliable, and valuable tool to identify 54% of NSM as newer potential pathogens of fungi causing ocular mycoses.
PURPOSE: To apply Polymerase Chain Reaction (PCR)-based DNA sequencing targeting Internal Transcribed Spacer (ITS) region for identification of non-sporulating molds (NSM) to species level which formed 12% of ocular isolates of fungi in a tertiary eye hospital in South India. MATERIALS AND METHODS: Fifty ocular filamentous fungal NSM isolates recovered from 45 patients were included in the study. PCR-based DNA sequencing technique targeting ITS region was applied to identify NSM. RESULTS: PCR-based DNA sequencing revealed 23 established pathogens involving 8 genera (Aspergillus, Fusarium, Bipolaris, Pythium, Cochilobolus, Exserohilum, Pseudoallescheria, and Scedosporiumspecies) and 27 emerging pathogens involving 7 genera (Botryosphaeria Lasiodiplodia species, Thielavia tortuosa, Glomerulla singulata, Macrophomina phaseolina, Rhizoctonia bataticola, and Podosporaspecies) reported for the first time in literature related to ocular infections. Fifteen (30%) patients with fungal keratitis caused by NSM failed to respond to standard antifungal therapy. CONCLUSION: PCR-based DNA sequencing technique is a rapid, reliable, and valuable tool to identify 54% of NSM as newer potential pathogens of fungi causing ocular mycoses.
Authors: Ashok Srinivasan; Brian L Wickes; Anna M Romanelli; Larisa Debelenko; Jeffrey E Rubnitz; Deanna A Sutton; Elizabeth H Thompson; Annette W Fothergill; Michael G Rinaldi; Randall T Hayden; Jerry L Shenep Journal: J Clin Microbiol Date: 2009-04-22 Impact factor: 5.948
Authors: K C da Cunha; D A Sutton; A W Fothergill; J Cano; J Gené; H Madrid; S De Hoog; P W Crous; J Guarro Journal: J Clin Microbiol Date: 2012-10-10 Impact factor: 5.948
Authors: Daniel W C L Santos; Ana Carolina B Padovan; Analy S A Melo; Sarah S Gonçalves; Viviane R Azevedo; Marilia M Ogawa; Tainá Veras Sandes Freitas; Arnaldo L Colombo Journal: Mycopathologia Date: 2013-01-04 Impact factor: 2.574
Authors: Maria V Castelli; Maria J Buitrago; Leticia Bernal-Martinez; Alicia Gomez-Lopez; Juan L Rodriguez-Tudela; Manuel Cuenca-Estrella Journal: J Clin Microbiol Date: 2008-08-06 Impact factor: 5.948