Literature DB >> 1826684

Isoform switching of the sarco(endo)plasmic reticulum Ca2+ pump during differentiation of BC3H1 myoblasts.

H de Smedt1, J A Eggermont, F Wuytack, J B Parys, L Van den Bosch, L Missiaen, J Verbis, R Casteels.   

Abstract

We have studied the expression of the gene 2 for the sarco(endo)plasmic reticulum Ca2+ pump (SERCA2) in BC3H1 cells. Myogenic differentiation not only activated the SERCA2 expression but it also induced an isoform switch. Undifferentiated myoblasts only expressed the SERCA2b isoform (non-muscle) whereas differentiated myocytes predominantly contained the SERCA2a isoform (cardiac/slow skeletal muscle). The isoform switch was documented by immunoblot analysis with isoform-specific antibodies. This observation was confirmed at the mRNA level by using antisense RNA probes specific for class 1 (SERCA2a) or class 2 (SERCA2b) messengers. The expression of the SERCA2a isoform after differentiation was accompanied by a decreased sensitivity of the Ca2+ uptake in permeabilized cells to the Ca2+ pump inhibitor thapsigargin.

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Year:  1991        PMID： 1826684

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  12 in total

1.  Functional difference between SERCA2a and SERCA2b Ca2+ pumps and their modulation by phospholamban.

Authors:  H Verboomen; F Wuytack; H De Smedt; B Himpens; R Casteels
Journal:  Biochem J       Date:  1992-09-01       Impact factor: 3.857

2.  Myostatin levels in regenerating rat muscles and in myogenic cell cultures.

Authors:  L Mendler; E Zádor; M Ver Heyen; L Dux; F Wuytack
Journal:  J Muscle Res Cell Motil       Date:  2000       Impact factor: 2.698

3.  Sequence elements surrounding the acceptor site suppress alternative splicing of the sarco/endoplasmic reticulum Ca2+-ATPase 2 gene transcript.

Authors:  L Van Den Bosch; L Mertens; S Gijsbers; M V Heyen; F Wuytack; J Eggermont
Journal:  Biochem J       Date:  1997-03-15       Impact factor: 3.857

4.  Alternative processing of the sarco/endoplasmic reticulum Ca(2+)-ATPase transcripts during muscle differentiation is a specifically regulated process.

Authors:  L Van den Bosch; L Mertens; Y Cavaloc; M Peterson; F Wuytack; J Eggermont
Journal:  Biochem J       Date:  1996-08-01       Impact factor: 3.857

5.  Differential expression and regulation of ryanodine receptor and myo-inositol 1,4,5-trisphosphate receptor Ca2+ release channels in mammalian tissues and cell lines.

Authors:  J J Mackrill; R A Challiss; D A O'connell; F A Lai; S R Nahorski
Journal:  Biochem J       Date:  1997-10-01       Impact factor: 3.857

6.  Mobilization of Ca2+ by thapsigargin and 2,5-di-(t-butyl)-1,4-benzohydroquinone in permeabilized insulin-secreting RINm5F cells: evidence for separate uptake and release compartments in inositol 1,4,5-trisphosphate-sensitive Ca2+ pool.

Authors:  M S Islam; P O Berggren
Journal:  Biochem J       Date:  1993-07-15       Impact factor: 3.857

7.  Ca2+ increase and Ca(2+)-influx in human tracheal smooth muscle cells: role of Ca2+ pools controlled by sarco-endoplasmic reticulum Ca(2+)-ATPase 2 isoform.

Authors:  Y Amrani; C Magnier; J Enouf; F Wuytack; C Bronner
Journal:  Br J Pharmacol       Date:  1995-08       Impact factor: 8.739

8.  Expression of phospholamban in C2C12 cells and regulation of endogenous SERCA1 activity.

Authors:  J M Harrer; S Ponniah; D G Ferguson; E G Kranias
Journal:  Mol Cell Biochem       Date:  1995-05-10       Impact factor: 3.396

9.  Spontaneously hypertensive rats and platelet Ca(2+)-ATPases: specific up-regulation of the 97 kDa isoform.

Authors:  B Papp; E Corvazier; C Magnier; T Kovàcs; N Bourdeau; S Lévy-Tolédano; R Bredoux; B Lévy; P Poitevin; A M Lompré
Journal:  Biochem J       Date:  1993-11-01       Impact factor: 3.857

10.  Regulation of splicing is responsible for the expression of the muscle-specific 2a isoform of the sarco/endoplasmic-reticulum Ca(2+)-ATPase.

Authors:  L Van den Bosch; J Eggermont; H De Smedt; L Mertens; F Wuytack; R Casteels
Journal:  Biochem J       Date:  1994-09-01       Impact factor: 3.857
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