Literature DB >> 18265370

Techniques for mammalian cell tissue culture.

Mary C Phelan1.   

Abstract

This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.

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Year:  2006        PMID： 18265370     DOI： 10.1002/0471142727.mba03fs74

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  5 in total

1.  Mouse models of human bladder cancer as a tool for drug discovery.

Authors:  Catherine Seager; Anna M Puzio-Kuter; Carlos Cordon-Cardo; James McKiernan; Cory Abate-Shen
Journal:  Curr Protoc Pharmacol       Date:  2010-06

2.  Real-time PCR assay is superior to other methods for the detection of mycoplasma contamination in the cell lines of the National Cell Bank of Iran.

Authors:  Vahid Molla Kazemiha; Shahin Bonakdar; Amir Amanzadeh; Shahram Azari; Arash Memarnejadian; Shirin Shahbazi; Mohammad Ali Shokrgozar; Reza Mahdian
Journal:  Cytotechnology       Date:  2015-03-06       Impact factor: 2.058

3.  Whole-cell patch-clamp analysis of recombinant NMDA receptor pharmacology using brief glutamate applications.

Authors:  Nathan G Glasgow; Jon W Johnson
Journal:  Methods Mol Biol       Date:  2014

4.  Revealing neuronal circuitry using stem cell-derived neurons.

Authors:  Isabella Garcia; Cynthia Kim; Benjamin R Arenkiel
Journal:  Curr Protoc Stem Cell Biol       Date:  2013

5.  Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9.

Authors:  Christopher J Giuliano; Ann Lin; Vishruth Girish; Jason M Sheltzer
Journal:  Curr Protoc Mol Biol       Date:  2019-09
  5 in total

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